Variantes genéticas rs1800629 en TNF y rs2228145 en IL6R: asociación con eventos supuestamente atribuibles a la vacunación e inmunización (ESAVI) y anticuerpos neutralizantes contra SARS-CoV-2 en la población del occidente de México vacunada con AZD1222

Abstract

Introduction

In this research, we evaluated the association of genetic variants rs1800629 in TNF and rs2228145 in IL6R with production of neutralizing antibodies against SARS-CoV-2 and the frequency of adverse effects following immunization (AEFIs) in adult population from Western of Mexico that received AZD1222 vaccination.

Methods

One hundred-seventeen adults were evaluated (33 years [23-40], 65% women). The self-reported frequency of AEFIs was recorded and the percentage of post-vaccination neutralizing antibodies was quantified. The identification of rs1800629 variant in TNF and rs2228145 in IL6R was performed by qPCR.

Results

The genotype frequencies of rs1800629 variant were: GG (86%) and GA (14%), and for rs2228145 variant were: AA (20%), CA (48%) and CC (32%). The percentage of post-vaccination antibodies was similar between men and women (median, 97.24%). An association was found between the frequency of AEFIs with the sex; being adynamia (p = 0.0243), chills (p = 0.0085), arthralgia (p = 0.0227) and pain in application area (p = 0.0096) more frequent in women. The GA genotype of rs1800629 variant showed an association with fever (p = 0.0131) and arthralgia (p = 0.0058) post-vaccination, but no relationship was found with the production of neutralizing antibodies after AZD1222 vaccination. The rs2228145 variant was not associated with the production of antibodies nor with the AEFIs reported.

Conclusion

The genetic variants rs1800629 in TNF and rs222815 in IL6R are not associated with the production of neutralizing antibodies against SARS-CoV-2 after receiving AZD1222 scheme in population from western of Mexico; however, the results suggest that rs1800629 variant increases the frequency of post-vaccination events, particularly fever and arthralgia.