Sex determination by ultrasonography prior to artificial breeding of the pufferfish Arothron manilensis (Tetraodontiformes, Tetraodontidae).

IF 1.2 4区 生物学 Q3 VETERINARY SCIENCES Zoo Biology Pub Date : 2024-05-01 Epub Date: 2024-01-31 DOI:10.1002/zoo.21822
Hiroyuki Doi, Shoki Murakami, Kazuyuki Momota, Hiroshi Obata, Harumi Sakai
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Abstract

Artificial breeding was induced in the pufferfish Arothron manilensis following ultrasonographic sex determination. Hormonal treatment of mature male and female specimens followed the collection (and measurement) of fully developed eggs by cannulation. Fertilized eggs (0.85 ± 0.02 mm diameter) were spherical, demersal and individually adhesive. Hatching occurred 5 days after fertilization, larvae being 2.23 ± 0.15 mm in total length and 2.08 ± 0.14 mm in notochord length. The larvae had all died within 14 days of hatching. To improve artificial breeding techniques for A. manilensis, it is necessary to determine more appropriate timing for hormone injection, as well as feeding nutrient-enhanced SS type Brachionus sp. to newly hatched larvae.

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河豚人工繁殖前的超声波性别鉴定。
通过超声波性别鉴定诱导河豚人工繁殖。对成熟的雌雄标本进行激素处理后,通过插管收集(和测量)发育完全的卵。受精卵(直径为 0.85 ± 0.02 毫米)呈球形,底栖并单独粘附。受精后 5 天孵化,幼虫全长 2.23 ± 0.15 毫米,脊索长 2.08 ± 0.14 毫米。幼虫在孵化后 14 天内全部死亡。为了改进马尼拉金枪鱼的人工繁殖技术,有必要确定更合适的激素注射时间,以及给刚孵化的幼体喂食营养强化的 SS 型 Brachionus sp.。
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来源期刊
Zoo Biology
Zoo Biology 生物-动物学
CiteScore
2.50
自引率
15.40%
发文量
85
审稿时长
6-12 weeks
期刊介绍: Zoo Biology is concerned with reproduction, demographics, genetics, behavior, medicine, husbandry, nutrition, conservation and all empirical aspects of the exhibition and maintenance of wild animals in wildlife parks, zoos, and aquariums. This diverse journal offers a forum for effectively communicating scientific findings, original ideas, and critical thinking related to the role of wildlife collections and their unique contribution to conservation.
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