Identification of qGL4.1 and qGL4.2, two closely linked QTL controlling grain length in rice

IF 2.6 3区农林科学 Q1 AGRONOMY Molecular Breeding Pub Date : 2024-01-30 DOI:10.1007/s11032-024-01447-y

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Abstract

Grain size is an important appearance quality trait in rice, which also affects grain yield. In this study, a recombinant inbred line (RIL) population derived from a cross between indica variety 9311 and japonica variety Cypress was constructed. And 181 out of 600 RILs were sequenced, and a high-density genetic map containing 2842 bin markers was constructed, with a total map length of 1500.6 cM. A total of 10 quantitative trait loci (QTL) related to grain length (GL), grain width (GW), grain length-to-width ratio (LWR), and 1000-grain weight (TGW) were detected under two environments. The genetic effect of qGL4, a minor QTL for GL and TGW, was validated using three heterogeneous inbred family (HIF) segregation populations. It was further dissected into two closed linked QTL, qGL4.1 and qGL4.2. By progeny testing, qGL4.1 and qGL4.2 were successfully delimited to intervals of 1304-kb and 423-kb, respectively. Our results lay the foundation for the map-based cloning of qGL4.1 and qGL4.2 and provide new gene resources for the improvement of grain yield and quality in rice.

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鉴定 qGL4.1 和 qGL4.2--控制水稻谷粒长度的两个密切相关的 QTL

摘要谷粒大小是水稻的一个重要外观品质性状，同时也影响谷粒产量。本研究构建了一个由籼稻品种 9311 和粳稻品种柏树杂交产生的重组近交系（RIL）群体。对 600 个 RIL 中的 181 个进行了测序，构建了包含 2842 个二进制标记的高密度遗传图谱，图谱总长度为 1500.6 cM。在两种环境下，共检测到与粒长（GL）、粒宽（GW）、粒长宽比（LWR）和千粒重（TGW）相关的10个数量性状位点（QTL）。利用三个异源近交系（HIF）分离群体验证了 qGL4 的遗传效应，它是 GL 和 TGW 的次要 QTL。它被进一步分解为两个封闭连接的 QTL，即 qGL4.1 和 qGL4.2。通过后代测试，qGL4.1 和 qGL4.2 分别被成功地划分为 1304-kb 和 423-kb 的区间。我们的研究结果为基于图谱克隆qGL4.1和qGL4.2奠定了基础，为提高水稻谷粒产量和品质提供了新的基因资源。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.