Museomics, molecular phylogeny and systematic revision of the Eurepini crickets (Orthoptera: Gryllidae: Eneopterinae), with description of two new genera

IF 4.7 1区 农林科学 Q1 ENTOMOLOGY Systematic Entomology Pub Date : 2024-01-29 DOI:10.1111/syen.12622
Shilin He, You Ning Su, Ming Kai Tan, Andreas Zwick, Ben H. Warren, Tony Robillard
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Abstract

Natural history collections worldwide house billions of specimens, representing one of the most globally important biobanks. In recent years, the advent of next-generation sequencing has significantly reduced the challenges of obtaining considerable genetic information from historical museum specimens. Crickets in the Australian tribe Eurepini Robillard are a good example of a taxon in which such museomic data have particularly strong potential to advance systematic knowledge, because comprehensive sampling requires decades of work over a very wide area. The tribe currently comprises 64 described species in five genera. Previous studies conflict in the generic relationships inferred for this tribe, all of which are poorly resolved, being based on limited data and sampling. In addition, there has so far been no systematic research for this tribe with extensive taxon sampling, and therefore, the consequence for genus boundaries remains to be investigated. To investigate phylogenetic relationships within Eurepini, we first applied the genome skimming approach to obtain molecular data from a comprehensive sample of Eurepini museum specimens. Of the 69 specimens sampled representing 61 described species, mainly including holotype specimens, we obtained 50 complete and 11 partially complete mitogenomes. Three nuclear genes (H3, 18S, and 28S) were also partially recovered for nearly all of these specimens. Phylogenetic analyses performed with mitogenomes plus three nuclear genes using maximum likelihood and Bayesian inference generated well-supported and highly congruent topologies. Eurepini was strongly recovered monophyletic with eight well-defined groups. These groups are used to revise the systematics of the tribe based on a combination of molecular phylogenetics and morphology. The phylogenetic results support the current definition of three genera (Eurepa Walker, Arilpa Otte & Alexander and Eurepella Otte & Alexander), lead us to redefine three genera (Salmanites Chopard, Napieria Baehr and Piestodactylus Saussure), and define and describe two new genera: Miripella Robillard, Tan & Su gen.nov. and Arrakis Robillard, Tan & Su gen.nov. Our results reinforce the importance of natural history collections as a repository for information on biodiversity and genetics, and provide the first comprehensive and robust phylogenetic framework for future systematic and evolutionary studies of Eurepini.

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Eurepini蟋蟀(直翅目:蝼蛄科:Eneopterinae)的缪斯组学、分子系统发育和系统修订,以及两个新属的描述
世界各地的自然历史收藏馆拥有数十亿件标本,是全球最重要的生物库之一。近年来,下一代测序技术的出现大大降低了从博物馆历史标本中获取大量遗传信息的难度。澳大利亚 Eurepini Robillard 部族中的蟋蟀就是一个很好的例子,在这个类群中,博物馆标本数据尤其具有推动系统知识的潜力,因为全面取样需要在非常广泛的区域内进行数十年的工作。该族目前有 5 属 64 个已描述的物种。以往的研究在推断该族的属种关系方面存在冲突,由于数据和取样有限,所有属种关系都没有得到很好的解决。此外,迄今为止还没有对该族进行过广泛的分类群取样的系统研究,因此对属界的影响仍有待调查。为了研究 Eurepini 的系统发育关系,我们首先采用了基因组掠取法,从 Eurepini 博物馆标本的综合样本中获取分子数据。在代表 61 个已描述物种(主要包括主模式标本)的 69 个样本中,我们获得了 50 个完整的有丝分裂基因组和 11 个部分完整的有丝分裂基因组。几乎所有这些标本的三个核基因(H3、18S 和 28S)也得到了部分恢复。使用最大似然法和贝叶斯推断法对有丝分裂基因组和三个核基因进行系统发育分析,得出了支持度高、高度一致的拓扑结构。Eurepini 强烈地恢复了单系,有八个明确的类群。根据分子系统学和形态学的结合,这些群组被用来修订该族的系统学。系统发生学的结果支持目前对三个属(Eurepa Walker、Arilpa Otte & Alexander 和 Eurepella Otte & Alexander)的定义,使我们重新定义了三个属(Salmanites Chopard、Napieria Baehr 和 Piestodactylus Saussure),并定义和描述了两个新属:Miripella Robillard, Tan & Su gen.nov. 和 Arrakis Robillard, Tan & Su gen.nov.我们的研究结果加强了自然历史藏品作为生物多样性和遗传学信息库的重要性,并为未来Eurepini的系统和进化研究提供了第一个全面而稳健的系统发育框架。
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来源期刊
Systematic Entomology
Systematic Entomology 生物-进化生物学
CiteScore
10.50
自引率
8.30%
发文量
49
审稿时长
>12 weeks
期刊介绍: Systematic Entomology publishes original papers on insect systematics, phylogenetics and integrative taxonomy, with a preference for general interest papers of broad biological, evolutionary or zoogeographical relevance.
期刊最新文献
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