Induction of the Cholinergic Phenotype in Mouse Neuroblastoma Cells Using Nerve Growth Factor

IF 0.5 4区医学 Q4 NEUROSCIENCES Neurochemical Journal Pub Date : 2023-12-01 DOI:10.1134/s181971242304013x

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Abstract—Nerve growth factor (NGF) is a factor which determines neuronal differentiation. NGF plays an important role in growth and differentiation of sensory and sympathetic neurons in the peripheral nervous system. In the mature brain, NGF is involved in the maintenance of the cholinergic neuronal phenotype. Here, we studied a possibility to induce the cholinergic phenotype in mouse neuroblastoma cells, which are often used to model various physiological and pathological processes occurring in the nervous system. Cells of NB41A3 and Neuro2a neuroblastoma lines are most frequently used to study the properties of cholinergic neurons. In the cells of these lines, the expression of TrkA and p75NGFR receptors, which is specific for the forebrain cholinergic nuclei, was revealed. Differentiation of the cells was induced by application of NGF or 8-Br-cAMP. NGF did not induce neuronal differentiation. Moreover, we did not find any changes in the content of choline acetyltransferase and vesicular acetylcholine transporter mRNA and protein, which were used as markers of the cholinergic phenotype. Thus, NB41A3 and Neuro2a cell lines cannot be advised as a model of cholinergic neurons in vitro because they do not differentiate and/or exhibit signs of the cholinergic phenotype in response to NGF stimulation.

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利用神经生长因子诱导小鼠神经母细胞瘤细胞的胆碱能表型

摘要-神经生长因子（NGF）是一种决定神经元分化的因子。NGF 在外周神经系统感觉神经元和交感神经元的生长和分化过程中发挥着重要作用。在成熟的大脑中，NGF 参与胆碱能神经元表型的维持。在这里，我们研究了在小鼠神经母细胞瘤细胞中诱导胆碱能表型的可能性，这种细胞经常被用来模拟神经系统中发生的各种生理和病理过程。NB41A3和Neuro2a神经母细胞瘤细胞系最常用来研究胆碱能神经元的特性。在这些品系的细胞中，发现了前脑胆碱能核特异性的TrkA和p75NGFR受体的表达。应用 NGF 或 8-Br-cAMP 可诱导细胞分化。NGF不能诱导神经元分化。此外，我们也没有发现胆碱能表型的标志物--胆碱乙酰转移酶和囊泡乙酰胆碱转运体 mRNA 和蛋白质的含量有任何变化。因此，NB41A3 和 Neuro2a 细胞系不能作为胆碱能神经元的体外模型，因为它们在 NGF 刺激下不会分化和/或表现出胆碱能表型的迹象。

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来源期刊

Neurochemical Journal 医学-神经科学

自引率

20.00%

发文量

审稿时长

>12 weeks

期刊介绍： Neurochemical Journal (Neirokhimiya) provides a source for the communication of the latest findings in all areas of contemporary neurochemistry and other fields of relevance (including molecular biology, biochemistry, physiology, neuroimmunology, pharmacology) in an afford to expand our understanding of the functions of the nervous system. The journal presents papers on functional neurochemistry, nervous system receptors, neurotransmitters, myelin, chromaffin granules and other components of the nervous system, as well as neurophysiological and clinical aspects, behavioral reactions, etc. Relevant topics include structure and function of the nervous system proteins, neuropeptides, nucleic acids, nucleotides, lipids, and other biologically active components. The journal is devoted to the rapid publication of regular papers containing the results of original research, reviews highlighting major developments in neurochemistry, short communications, new experimental studies that use neurochemical methodology, descriptions of new methods of value for neurochemistry, theoretical material suggesting novel principles and approaches to neurochemical problems, presentations of new hypotheses and significant findings, discussions, chronicles of congresses, meetings, and conferences with short presentations of the most sensational and timely reports, information on the activity of the Russian and International Neurochemical Societies, as well as advertisements of reagents and equipment.