Dry storage alters intraspecific variation in phenotypic traits at early life stages: evidence from a dominant alpine meadow species

Abstract

The intraspecific variations of phenotypic traits in the early life stages, such as seed germination and seedling establishment, are important components affecting species adaptation and differentiation. As one of the most common ways in which seeds are maintained, dry storage usually affects these traits, by either increasing or decreasing variation among populations. However, little is known about how the interaction between dry storage and population variation of a species affects the performance and adaptability of early life-cycle traits. In this study, we conducted experiments with seeds from ten populations of Elymus nutans along an altitudinal gradient on the eastern Qinghai-Tibet Plateau. Fresh seeds and seeds stored dry for 6 months were used to quantify the temperature thresholds for germination, determine seedling emergence and survival in two common gardens and examine the correlation between these traits and environmental conditions of population provenance. Dry storage increased germination percentage, germination speed and seedling emergence, and decreased intraspecific variation in germination traits of seeds (reduced by 33.36, 52.05 and 20.45% for Tb, θT(50) and σθT, respectively). Dry storage had little effect on the intraspecific variation of seedling emergence and survival. In addition, the temperature threshold for germination cannot be used to predict seedling emergence and survival in either common garden, regardless of whether seeds were stored or not. These results indicated that it is feasible to use dry-stored seeds to evaluate and select suitable provenances in ecological restoration, and using dry-stored seeds in ecological restoration projects is expected to achieve better vegetation restoration results than fresh seeds. On the other hand, the status of seeds (fresh vs. dry stored) should be considered in an evaluation of the adaptive value of plant functional traits, especially in the early life stages, otherwise, inconsistent conclusions may be drawn.