Impacts of electrochemical disinfection on the viability and structure of the microbiome in secondary effluent water

IF 6.1 2区环境科学与生态学 Q2 ENGINEERING, ENVIRONMENTAL Frontiers of Environmental Science & Engineering Pub Date : 2024-01-26 DOI:10.1007/s11783-024-1818-1

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Abstract

Electrochemical disinfection (ECD) is a promising disinfection technique for wastewater reclamation; however, the impacts of ECD on the microbiome in secondary effluent wastewater remain unknown. In this study, Propidium monoazide-qPCR (PMA-qPCR) and the plate count method were used to evaluate the inactivation performance, and the PMA-16S rRNA gene sequences of living cells were targeted to study the microbiome. A discrepancy was found between PMA-qPCR and the plate count method in the evaluation of cell count, with increases of 1.5 to 2.2 orders of magnitude in the disinfection rate after 150 s of disinfection. However, the cell count recovered and occasionally exceeded original levels within 3 d after disinfection. Biodiversity was suppressed after ECD, but the microbiome after 150 s disinfection retained a higher level of evenness and stability in the community with a median Shannon index (> 3.7). Pathogenic bacteria remained high in relative abundance even after 150 s of 25 V disinfection, but the biofilm-forming population was effectively suppressed by ECD. The co-occurrence network revealed a centralized and fragile network as disinfection persisted, demonstrating the destabilizing effects of ECD on the microbiome. Functional pathways for cell membrane synthesis and organic compound degradation were enriched after ECD. The reaction of the microbiome after ECD was similar to other disinfection techniques in terms of community structure.

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电化学消毒对二级污水中微生物群活力和结构的影响

摘要电化学消毒（ECD）是一种前景广阔的废水再生消毒技术，然而，ECD 对二级出水废水中微生物组的影响仍然未知。本研究采用丙啶单氮qPCR（PMA-qPCR）和平板计数法评估灭活性能，并以活细胞的 PMA-16S rRNA 基因序列为目标研究微生物组。发现 PMA-qPCR 和平板计数法在评估细胞数方面存在差异，消毒 150 秒后，消毒率增加了 1.5 至 2.2 个数量级。不过，细胞数在消毒后 3 天内恢复并偶尔超过原来的水平。生物多样性在 ECD 之后受到抑制，但经过 150 秒消毒后的微生物群落保持了较高的均匀度和群落稳定性，香农指数中位数（> 3.7）。即使经过 150 秒的 25 V 消毒，致病菌的相对丰度仍然很高，但生物膜形成种群在 ECD 的作用下被有效抑制。随着消毒的持续，共现网络显示出一个集中而脆弱的网络，这表明了 ECD 对微生物组的不稳定影响。细胞膜合成和有机化合物降解的功能途径在 ECD 后得到了丰富。就群落结构而言，ECD 后微生物群的反应与其他消毒技术相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers of Environmental Science & Engineering ENGINEERING, ENVIRONMENTAL-ENVIRONMENTAL SCIENCES

CiteScore

10.90

自引率

12.50%

发文量

988

审稿时长

6.1 months

期刊介绍： Frontiers of Environmental Science & Engineering (FESE) is an international journal for researchers interested in a wide range of environmental disciplines. The journal''s aim is to advance and disseminate knowledge in all main branches of environmental science & engineering. The journal emphasizes papers in developing fields, as well as papers showing the interaction between environmental disciplines and other disciplines. FESE is a bi-monthly journal. Its peer-reviewed contents consist of a broad blend of reviews, research papers, policy analyses, short communications, and opinions. Nonscheduled “special issue” and "hot topic", including a review article followed by a couple of related research articles, are organized to publish novel contributions and breaking results on all aspects of environmental field.