Comprehensive whole-genome sequencing reveals genetic characteristics of Colletotrichum fructicola (Nara gc5) the causative organism of circular leaf spot disease of rubber (Hevea brasiliensis)

IF 2.2 4区农林科学 Q2 PLANT SCIENCES Journal of Plant Pathology Pub Date : 2024-02-03 DOI:10.1007/s42161-023-01578-9

{"title":"Comprehensive whole-genome sequencing reveals genetic characteristics of Colletotrichum fructicola (Nara gc5) the causative organism of circular leaf spot disease of rubber (Hevea brasiliensis)","authors":"","doi":"10.1007/s42161-023-01578-9","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> Hevea brasiliensis (Para rubber tree) is a commercially cultivated crop for latex production worldwide. Rubber trees from Southern states of India have been affected by Circular Leaf Spot (CLS) disease caused by Colletotrichum fructicola and Colletotrichum siamense causing yellowing and severe defoliation in rubber plantations in recent years. In this study, we collected infected leaf samples of CLS disease from different locations in the South Indian states of Kerala, Tamil Nadu, and Karnataka. Colletotrichum fructicola and C. siamense were identified as the causative agents of CLS disease and the isolate, C. fructicola was used for genomics analysis. We performed whole-genome sequencing (WGS) of, C. fructicola and carried out gene content analysis by comparing it with other reported Colletotrichum species. In silico analysis revealed a rich repertoire of pathogenicity genes and CAZymes in C. fructicola encoding secreted proteins, effectors, plant cell wall degrading enzymes, and secondary metabolism-associated proteins with possible roles in the host-specific interaction and pathogenicity. Our dataset provides a rich resource for understanding the pathogenicity-related genes that are involved in CLS disease progression and gives a basis for subsequent functional genomics research in C. fructicola.","PeriodicalId":16837,"journal":{"name":"Journal of Plant Pathology","volume":"85 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s42161-023-01578-9","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Hevea brasiliensis (Para rubber tree) is a commercially cultivated crop for latex production worldwide. Rubber trees from Southern states of India have been affected by Circular Leaf Spot (CLS) disease caused by Colletotrichum fructicola and Colletotrichum siamense causing yellowing and severe defoliation in rubber plantations in recent years. In this study, we collected infected leaf samples of CLS disease from different locations in the South Indian states of Kerala, Tamil Nadu, and Karnataka. Colletotrichum fructicola and C. siamense were identified as the causative agents of CLS disease and the isolate, C. fructicola was used for genomics analysis. We performed whole-genome sequencing (WGS) of, C. fructicola and carried out gene content analysis by comparing it with other reported Colletotrichum species. In silico analysis revealed a rich repertoire of pathogenicity genes and CAZymes in C. fructicola encoding secreted proteins, effectors, plant cell wall degrading enzymes, and secondary metabolism-associated proteins with possible roles in the host-specific interaction and pathogenicity. Our dataset provides a rich resource for understanding the pathogenicity-related genes that are involved in CLS disease progression and gives a basis for subsequent functional genomics research in C. fructicola.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

全基因组综合测序揭示了橡胶（Hevea brasiliensis）圆叶斑病病原菌 Colletotrichum fructicola (Nara gc5) 的遗传特征

Abstract Hevea brasiliensis（顺丁橡胶树）是世界范围内生产乳胶的商业化种植作物。近年来，印度南部各邦的橡胶树受到由Colletotrichum fructicola和Colletotrichum siamense引起的圆叶斑病（CLS）的影响，导致橡胶种植园枯黄和严重落叶。在这项研究中，我们从南印度喀拉拉邦、泰米尔纳德邦和卡纳塔克邦的不同地点采集了感染 CLS 病害的叶片样本。结果表明，Colletotrichum fructicola 和 C. siamense 是 CLS 病害的致病菌，其中 C. fructicola 分离物被用于基因组学分析。我们对 C. fructicola 进行了全基因组测序（WGS），并将其与其他已报道的 Colletotrichum 种类进行了比较，从而进行了基因含量分析。硅学分析揭示了果蝇C.中丰富的致病基因和CAZymes，这些基因编码分泌蛋白、效应蛋白、植物细胞壁降解酶和次生代谢相关蛋白，可能在宿主特异性相互作用和致病性中发挥作用。我们的数据集为了解参与 CLS 疾病进展的致病性相关基因提供了丰富的资源，并为果蝇的后续功能基因组学研究奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Plant Pathology 生物-植物科学

CiteScore

3.10

自引率

4.50%

发文量

218

审稿时长

6-12 weeks

期刊介绍： The Journal of Plant Pathology (JPP or JPPY) is the main publication of the Italian Society of Plant Pathology (SiPAV), and publishes original contributions in the form of full-length papers, short communications, disease notes, and review articles on mycology, bacteriology, virology, phytoplasmatology, physiological plant pathology, plant-pathogeninteractions, post-harvest diseases, non-infectious diseases, and plant protection. In vivo results are required for plant protection submissions. Varietal trials for disease resistance and gene mapping are not published in the journal unless such findings are already employed in the context of strategic approaches for disease management. However, studies identifying actual genes involved in virulence are pertinent to thescope of the Journal and may be submitted. The journal highlights particularly timely or novel contributions in its Editors’ choice section, to appear at the beginning of each volume. Surveys for diseases or pathogens should be submitted as "Short communications".