Extraction and Characterization of Beta-glucan Methyl Esters Derived From UV-mutated Marine Yeast and their Applications in Cosmetics

Thalassas: An International Journal of Marine Sciences Pub Date : 2024-02-03 DOI:10.1007/s41208-024-00661-x

{"title":"Extraction and Characterization of Beta-glucan Methyl Esters Derived From UV-mutated Marine Yeast and their Applications in Cosmetics","authors":"","doi":"10.1007/s41208-024-00661-x","DOIUrl":null,"url":null,"abstract":"<h3>Abstract</h3> <p>Beta-glucan is a natural polysaccharide, abundantly present in the cell walls of several fungi, majorly in mushrooms, yeasts and many bacterial species. It can be used in the cosmetics industry as it has good anti-aging, wound healing and antioxidant properties. Marine yeasts were isolated from the backwater samples derived from Andaman-Nicobar Islands, India and screened for beta-glucan production. Among 38 yeast isolates, 5 isolates could produce beta-glucan and they were further chosen for UV mutagenesis at different time intervals for strain improvement. Mutated marine yeast isolate DAMB1 was found to be producing the highest amount of beta-glucan (1.67 gm/100 ml). Further, potential isolates were characterized microscopically and checked for their antioxidant activity and anti-bacterial activity against bacterial pathogens. The beta-glucan compounds exhibited good antioxidant activity as well as good antimicrobial activity against all the pathogens and highest against <em>Staphylococcus aureus</em> (23 ± 0.7 mm). The yeast-derived compound was structurally characterized using GCMS analysis and represented the presence of Ethylthiophosphonamide Acid, O-methyl Ester, and 1,2,4-Trithiolane which are highly pure fatty acid methyl esters. The extracted beta-glucan compound from potential yeast isolate was used to formulate beta-glucan almond scrub consisting of sweet almond oil and almond powder. The beta-glucan almond scrub showed no side effects or infection on the skin after a period of usage (10 weeks) rather it smoothened out the rough skin to an extent. Finally, the yeast was further characterized and identified as <em>Rhodotorula sp.</em> DAMB1 (Acc. No. MK968443) through 18s rRNA sequencing analysis.</p>","PeriodicalId":22298,"journal":{"name":"Thalassas: An International Journal of Marine Sciences","volume":"29 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Thalassas: An International Journal of Marine Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s41208-024-00661-x","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Beta-glucan is a natural polysaccharide, abundantly present in the cell walls of several fungi, majorly in mushrooms, yeasts and many bacterial species. It can be used in the cosmetics industry as it has good anti-aging, wound healing and antioxidant properties. Marine yeasts were isolated from the backwater samples derived from Andaman-Nicobar Islands, India and screened for beta-glucan production. Among 38 yeast isolates, 5 isolates could produce beta-glucan and they were further chosen for UV mutagenesis at different time intervals for strain improvement. Mutated marine yeast isolate DAMB1 was found to be producing the highest amount of beta-glucan (1.67 gm/100 ml). Further, potential isolates were characterized microscopically and checked for their antioxidant activity and anti-bacterial activity against bacterial pathogens. The beta-glucan compounds exhibited good antioxidant activity as well as good antimicrobial activity against all the pathogens and highest against Staphylococcus aureus (23 ± 0.7 mm). The yeast-derived compound was structurally characterized using GCMS analysis and represented the presence of Ethylthiophosphonamide Acid, O-methyl Ester, and 1,2,4-Trithiolane which are highly pure fatty acid methyl esters. The extracted beta-glucan compound from potential yeast isolate was used to formulate beta-glucan almond scrub consisting of sweet almond oil and almond powder. The beta-glucan almond scrub showed no side effects or infection on the skin after a period of usage (10 weeks) rather it smoothened out the rough skin to an extent. Finally, the yeast was further characterized and identified as Rhodotorula sp. DAMB1 (Acc. No. MK968443) through 18s rRNA sequencing analysis.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

从紫外线突变海洋酵母中提取β-葡聚糖甲酯及其在化妆品中的应用的提取和表征

摘要 β-葡聚糖是一种天然多糖，大量存在于几种真菌的细胞壁中，主要存在于蘑菇、酵母和许多细菌种类中。它具有良好的抗衰老、伤口愈合和抗氧化特性，可用于化妆品行业。从印度安达曼-尼科巴群岛的回水样本中分离出了海洋酵母菌，并对其生产的β-葡聚糖进行了筛选。在 38 个酵母分离物中，有 5 个分离物能产生β-葡聚糖，并进一步选择它们进行不同时间间隔的紫外诱变，以改良菌株。经诱变的海洋酵母分离物 DAMB1 被发现能产生最高量的β-葡聚糖（1.67 克/100 毫升）。此外，还对潜在的分离物进行了显微鉴定，并检查了它们的抗氧化活性和对细菌病原体的抗菌活性。β-葡聚糖化合物对所有病原体都表现出良好的抗氧化活性和抗菌活性，其中对金黄色葡萄球菌的抗菌活性最高（23 ± 0.7 mm）。利用 GCMS 分析法对酵母提取物进行了结构鉴定，结果表明其中含有乙硫基膦酰胺酸、O-甲基酯和 1,2,4-三硫环戊烷，它们都是高纯度的脂肪酸甲酯。从潜在酵母分离物中提取的 beta-葡聚糖化合物被用来配制由甜杏仁油和杏仁粉组成的 beta-葡聚糖杏仁磨砂膏。使用一段时间（10 周）后，β-葡聚糖杏仁磨砂膏对皮肤没有副作用或感染，反而在一定程度上抚平了粗糙的皮肤。最后，通过 18s rRNA 测序分析，进一步确定了该酵母菌为 Rhodotorula sp.

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Thalassas: An International Journal of Marine Sciences

自引率

0.00%

发文量