A. D. Burtseva, T. N. Baymukhametov, I. O. Ilyasov, M. A. Bolshakov, A. A. Moskalenko, K. M. Boyko, A. A. Ashikhmin
{"title":"Structural Study of the Light-Harvesting Complex LH2 from the Purple Sulfur Bacteria Ectothiorhodospira haloalkaliphila by Cryoelectronic Microscopy","authors":"A. D. Burtseva, T. N. Baymukhametov, I. O. Ilyasov, M. A. Bolshakov, A. A. Moskalenko, K. M. Boyko, A. A. Ashikhmin","doi":"10.1134/s1063774523601132","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>The primary processes of photosynthesis for purple photosynthesising bacteria occur in light-harvesting (LH) complexes. The LH2 complex contains polypeptides; bacteriochlorophyll; and, in most cases, carotenoids. There are three known spatial structures of LH2 complexes from purple nonsulfur bacteria; however, high-resolution structures have not been established for purple sulfur bacteria. The results of the structural study of two light-harvesting complexes LH2 from purple sulfur bacteria <i>Ectothiorhodospira haloalkaliphila</i> by cryoelectronic microscopy are reported. Images of carotenoid-containing (LH2+) and carotenoidless (LH2–) variants of the complex, demonstrating a characteristic architecture of the objects of this type, are obtained. A 3D reconstruction of LH2+ is performed with a resolution of 4.5 Å; it coincides with the previously established crystal structure. The presence of particles of different morphology is shown for LH2–.</p>","PeriodicalId":527,"journal":{"name":"Crystallography Reports","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crystallography Reports","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1134/s1063774523601132","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CRYSTALLOGRAPHY","Score":null,"Total":0}
引用次数: 0
Abstract
The primary processes of photosynthesis for purple photosynthesising bacteria occur in light-harvesting (LH) complexes. The LH2 complex contains polypeptides; bacteriochlorophyll; and, in most cases, carotenoids. There are three known spatial structures of LH2 complexes from purple nonsulfur bacteria; however, high-resolution structures have not been established for purple sulfur bacteria. The results of the structural study of two light-harvesting complexes LH2 from purple sulfur bacteria Ectothiorhodospira haloalkaliphila by cryoelectronic microscopy are reported. Images of carotenoid-containing (LH2+) and carotenoidless (LH2–) variants of the complex, demonstrating a characteristic architecture of the objects of this type, are obtained. A 3D reconstruction of LH2+ is performed with a resolution of 4.5 Å; it coincides with the previously established crystal structure. The presence of particles of different morphology is shown for LH2–.
期刊介绍:
Crystallography Reports is a journal that publishes original articles short communications, and reviews on various aspects of crystallography: diffraction and scattering of X-rays, electrons, and neutrons, determination of crystal structure of inorganic and organic substances, including proteins and other biological substances; UV-VIS and IR spectroscopy; growth, imperfect structure and physical properties of crystals; thin films, liquid crystals, nanomaterials, partially disordered systems, and the methods of studies.