mRNA Levels of Epithelial and Mesenchymal Markers in Lung Epithelial Cell Lines.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-07-01 DOI:10.61186/rbmb.12.2.211
Ekaterina Sergeevna Karetnikova, Natalia Jarzebska, Roman Nikolaevich Rodionov, Elena Rubets, Alexander Georgievich Markov, Peter Markus Spieth
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Abstract

Background: Epithelial-mesenchymal transition (EMT) is an important physiologic process that determines the outcome of lung tissue healing after injury. Stimuli and molecular cascades inducing EMT lead to up-regulation of the mesenchymal-specific genes in the alveolar epithelial cells and to down-regulation of the genes coding for epithelial markers. Alveolar epithelial cell lines are commonly used as in vitro models to study processes occurring in the lung tissue. The aim of this study is to quantify and compare mRNA expression levels of epithelial and mesenchymal markers in a number of lung epithelial cell lines.

Methods: Lung epithelial cell lines L2, R3/1 and RLE-6TN were cultured. Repeated mRNA isolation, reverse transcription, and quantitative PCR with primers to epithelial (E-cadherin, occludin, and ZO-2) and mesenchymal (α-SMA, collagen III, and vimentin) markers were performed.

Results: First, our study revealed a higher level of epithelial transcripts in the RLE-6TN cell line compared to L2 and R3/1 cells. Secondly, we have found simultaneous mRNA expression of both epithelial (E-cadherin, occludin and ZO-2) and mesenchymal (α-SMA, collagen III and vimentin) markers in all cell lines studied.

Conclusions: Our data indicate that at the transcriptional level the L2, R3/1, and RLE-6TN cell lines are at one of the intermediate stages of EMT, which opens new possibilities for the study of EMT on cell lines. Determination of the direction of changes in epithelial and mesenchymal markers will make it possible to establish the factors responsible for both EMT and reverse mesenchymal-epithelial transition.

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肺上皮细胞系中上皮和间质标志物的 mRNA 水平
背景:上皮-间质转化(EMT)是一个重要的生理过程,它决定着损伤后肺组织愈合的结果。诱导 EMT 的刺激和分子级联导致肺泡上皮细胞中间质特异性基因的上调和上皮标记基因的下调。肺泡上皮细胞系通常被用作研究肺组织过程的体外模型。本研究旨在量化和比较一些肺上皮细胞系中上皮和间质标志物的 mRNA 表达水平:方法:培养肺上皮细胞系 L2、R3/1 和 RLE-6TN。方法:对肺上皮细胞系 L2、R3/1 和 RLE-6TN 进行培养,用上皮标记物(E-cadherin、occludin 和 ZO-2)和间质标记物(α-SMA、胶原 III 和波形蛋白)引物重复进行 mRNA 分离、反转录和定量 PCR:结果:首先,我们的研究发现,与 L2 和 R3/1 细胞相比,RLE-6TN 细胞系的上皮细胞转录本水平更高。其次,我们发现在研究的所有细胞系中,上皮(E-cadherin、occludin 和 ZO-2)和间充质(α-SMA、胶原 III 和波形蛋白)标记物的 mRNA 同时表达:我们的数据表明,在转录水平上,L2、R3/1 和 RLE-6TN 细胞系处于 EMT 的中间阶段之一,这为研究细胞系的 EMT 提供了新的可能性。确定上皮和间质标志物的变化方向将有可能确定导致EMT和间质-上皮逆转的因素。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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