The Impact of Metformin on Dust-Induced Histopathological Changes and Oxidative Stress in the Liver: An Insight into Dust Concentration and Liver Biomarkers in Animal Models.

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Reports of Biochemistry and Molecular Biology Pub Date : 2023-07-01 DOI:10.61186/rbmb.12.2.306
Soheila Aminzadeh, Maryam Salehcheh, Mohammad Javad Khodayar, Gholamreza Goudarzi, Ali Asghar Hemmati, Laya Sadat Khorsandi, Nezam Asgharipour Dasht Bozorg
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Abstract

Background: Environmental pollution has a profound impact on both human and animal life. Khuzestan province, which has been plagued by intense dust storms and pollution for decades, is the focus of this study. The research aims to investigate the protective effects of metformin against the toxicity of particulate matter in the livers of rats.

Methods: Male Wistar rats were selected for the study and divided into six groups: a control group, Metformin-treated groups, Iraqi dust-exposed group (Iraqi-D), Local dust-exposed group (Local-D), Iraqi dust-exposed with Metformin treatment group (Iraqi-D+Metformin), and Local dust-exposed with Metformin treatment group (Local-D+Metformin). The rats were exposed to local and Iraqi dust through a nebulizer and received oral metformin for a duration of 21 days. At the end of the intervention, liver biomarkers and oxidative stress factors were evaluated enzymatically.

Results: The study revealed that rats exposed to Iraqi and local dust experienced a significant increase in liver biomarkers, including aspartate aminotransferase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALK) levels, alongside a decrease in glutathione (GSH) concentrations and an increase in malondialdehyde (MDA) levels. However, treatment with metformin was effective in preventing the increase in these biomarkers, restoring GSH levels, and averting the rise in MDA levels, as compared to the control group.

Conclusions: Exposure to particulate matter from Iraq and the local region can induce alterations in biomarkers and oxidative stress levels in the rat liver, and these effects can be mitigated through metformin treatment.

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二甲双胍对粉尘诱导的肝脏组织病理学变化和氧化应激的影响:洞察动物模型中的粉尘浓度和肝脏生物标志物
背景:环境污染对人类和动物的生活都有着深远的影响。几十年来,胡齐斯坦省一直饱受强烈沙尘暴和污染的困扰,本研究的重点就是该省。研究旨在探讨二甲双胍对大鼠肝脏颗粒物毒性的保护作用:研究选取雄性 Wistar 大鼠,将其分为六组:对照组、二甲双胍处理组、伊拉克粉尘暴露组(Iraqi-D)、本地粉尘暴露组(Local-D)、伊拉克粉尘暴露加二甲双胍处理组(Iraqi-D+二甲双胍)和本地粉尘暴露加二甲双胍处理组(Local-D+二甲双胍)。大鼠通过雾化器接触当地粉尘和伊拉克粉尘,并接受为期 21 天的二甲双胍口服治疗。干预结束后,对肝脏生物标志物和氧化应激因子进行酶学评估:研究发现,暴露于伊拉克和当地粉尘的大鼠的肝脏生物标志物(包括天门冬氨酸氨基转移酶(AST)、丙氨酸转氨酶(ALT)和碱性磷酸酶(ALK)水平)显著增加,同时谷胱甘肽(GSH)浓度降低,丙二醛(MDA)水平升高。然而,与对照组相比,二甲双胍治疗能有效防止这些生物标志物的增加,恢复GSH水平,并避免MDA水平的上升:结论:暴露于伊拉克和当地地区的微粒物质会引起大鼠肝脏中生物标志物和氧化应激水平的改变,而二甲双胍治疗可减轻这些影响。
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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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