Combined Training Improves Gene Expression Related to Immunosenescence in Obese Type 2 Diabetic Individuals.

Diego T Brunelli, Ivan L P Bonfante, Vinicius O Boldrini, Pedro G Scolfaro, Renata G Duft, Keryma Mateus, Raphael F Fatori, Mara P T Chacon-Mikahil, Alessandro S Farias, Ana M Teixeira, Cláudia R Cavaglieri
{"title":"Combined Training Improves Gene Expression Related to Immunosenescence in Obese Type 2 Diabetic Individuals.","authors":"Diego T Brunelli, Ivan L P Bonfante, Vinicius O Boldrini, Pedro G Scolfaro, Renata G Duft, Keryma Mateus, Raphael F Fatori, Mara P T Chacon-Mikahil, Alessandro S Farias, Ana M Teixeira, Cláudia R Cavaglieri","doi":"10.1080/02701367.2023.2299716","DOIUrl":null,"url":null,"abstract":"<p><p><b>Purpose:</b> The aim of this study was to investigate the effects of moderate combined training (CT) on both the gene expression of pro- and anti-inflammatory markers and senescence in the immune system in peripheral blood mononuclear cells (PBMCs) and subcutaneous adipose tissue (SAT) of obese middle-aged individuals with type 2 diabetes (T2D). <b>Methods:</b> Thirty obese individuals (50.2 ± 9.4 years; body mass index: 31.8 ± 2.3 kg/m²) with T2D underwent 16 weeks of a CT group [CT; aerobic (50-60% of VO2<sub>max</sub>) plus resistance (50-75% of 1RM) training; 3 times/week, 70 min/session; <i>n</i> = 16)] or a control group (CG, <i>n</i> = 14). Nutritional patterns, muscle strength (1RM), cardiorespiratory fitness (VO2<sub>max</sub>), waist circumference (WC), body composition (Air Displacement Plethysmograph) and blood collections for biochemical (serum leptin, IL-2, IL-4, IL-6, IL-10, TNF-α and anti-CMV) and molecular (gene expression of leptin, IL-2, IL-4, IL-6, IL-10, TNF-α, PD-1, P16<sup>ink4a</sup>, CCR7, CD28 and CD27 in PBMCs and SAT) analyses were assessed before (Pre) and after (Post) the 16 weeks of the experimental period. <b>Results:</b> Significant decreases were observed in WC and IL4, TNF-α, PD-1 and CD27 expression in PBMCs for CT. Furthermore, significant increases were observed in 1RM and VO2max for CT after the experimental period. <b>Conclusion:</b> Moderate CT contributed to a reduction in the gene expression of markers associated to chronic inflammation and immunosenescence in PBMCs of obese middle-aged individuals with T2D.</p>","PeriodicalId":94191,"journal":{"name":"Research quarterly for exercise and sport","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research quarterly for exercise and sport","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/02701367.2023.2299716","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/6 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Purpose: The aim of this study was to investigate the effects of moderate combined training (CT) on both the gene expression of pro- and anti-inflammatory markers and senescence in the immune system in peripheral blood mononuclear cells (PBMCs) and subcutaneous adipose tissue (SAT) of obese middle-aged individuals with type 2 diabetes (T2D). Methods: Thirty obese individuals (50.2 ± 9.4 years; body mass index: 31.8 ± 2.3 kg/m²) with T2D underwent 16 weeks of a CT group [CT; aerobic (50-60% of VO2max) plus resistance (50-75% of 1RM) training; 3 times/week, 70 min/session; n = 16)] or a control group (CG, n = 14). Nutritional patterns, muscle strength (1RM), cardiorespiratory fitness (VO2max), waist circumference (WC), body composition (Air Displacement Plethysmograph) and blood collections for biochemical (serum leptin, IL-2, IL-4, IL-6, IL-10, TNF-α and anti-CMV) and molecular (gene expression of leptin, IL-2, IL-4, IL-6, IL-10, TNF-α, PD-1, P16ink4a, CCR7, CD28 and CD27 in PBMCs and SAT) analyses were assessed before (Pre) and after (Post) the 16 weeks of the experimental period. Results: Significant decreases were observed in WC and IL4, TNF-α, PD-1 and CD27 expression in PBMCs for CT. Furthermore, significant increases were observed in 1RM and VO2max for CT after the experimental period. Conclusion: Moderate CT contributed to a reduction in the gene expression of markers associated to chronic inflammation and immunosenescence in PBMCs of obese middle-aged individuals with T2D.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
综合训练可改善肥胖的 2 型糖尿病患者与免疫衰老相关的基因表达。
目的:本研究旨在探讨适度的联合训练(CT)对肥胖的中年 2 型糖尿病(T2D)患者外周血单核细胞(PBMCs)和皮下脂肪组织(SAT)中促炎和抗炎标志物基因表达以及免疫系统衰老的影响。研究方法30 名患有 T2D 的肥胖者(50.2 ± 9.4 岁;体重指数:31.8 ± 2.3 kg/m²)参加了为期 16 周的 CT 组[CT;有氧(50-60% VO2max)加阻力(50-75% 1RM)训练;每周 3 次,每次 70 分钟;n = 16]或对照组(CG,n = 14)。营养模式、肌肉力量(1RM)、心肺功能(VO2max)、腰围(WC)、身体成分(空气置换压力计)和血液采集,以检测生化指标(血清瘦素、IL-2、IL-4、IL-6、IL-10、TNF-α和抗CMV)、和抗 CMV)和分子(PBMC 和 SAT 中瘦素、IL-2、IL-4、IL-6、IL-10、TNF-α、PD-1、P16ink4a、CCR7、CD28 和 CD27 的基因表达)分析。结果观察到 CT 的 WC 和 PBMCs 中 IL4、TNF-α、PD-1 和 CD27 的表达显著下降。此外,实验结束后,观察到 CT 的 1RM 和 VO2max 有明显增加。结论适度 CT 有助于减少患有 T2D 的肥胖中年人的 PBMC 中与慢性炎症和免疫衰老相关的标记物的基因表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
The Influence of Mental Fatigue on Physical Performance and Its Relationship with Rating Perceived Effort and Enjoyment in Older Adults. Greater Excess Post-Exercise Oxygen Consumption and Fat Use Following Calisthenics vs. Oxygen Consumption Matched Steady-State Exercise. Muscle Hypertrophy and Strength Adaptations to Systematically Varying Resistance Exercises. The Effect of Attention Focus Instructions on Strength and Balance in Subjects With Generalized Joint Hypermobility. Neural and Muscular Determinants of Performance Fatigability Are Independent of Work and Recovery Durations During High-Intensity Interval Exercise in Males.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1