Pleiotropic immunoregulation by growth-blocking peptide in Ostrinia furnacalis

IF 2.3 2区农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-02-08 DOI:10.1111/imb.12898

Dongchun Hu, Fuqiang Xu, Zupeng Gao, Kangkang Chen, Wenlong Guo, Zitian Wang, Shuzhong Li, Congjing Feng

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Abstract

Insects rely on their innate immune system to eliminate pathogenic microbes. As a system component, cytokines transmit intercellular signals to control immune responses. Growth-blocking peptide (GBP) is a member of the stress-responsive peptide family of cytokines found in several orders of insects, including Drosophila. However, the physiological role of GBP in defence against pathogens is not thoroughly understood. In this study, we explored the functions of GBP in a lepidopteran pest, Ostrinia furnacalis. Injection of recombinant O. furnacalis GBP (OfGBP) precursor (proGBP) and chemically synthesised GBP significantly induced the transcription of antimicrobial peptides (AMPs) and other immunity-related genes including immune deficiency (IMD) and Dorsal. The level of OfGBP mRNA was upregulated after bacterial infection. Knockdown of OfGBP expression led to a decrease in IMD, Relish, MyD88 and Dorsal mRNA levels. OfGBP induced phenoloxidase activity and affected hemocyte behaviours in O. furnacalis larvae. In summary, GBP is a potent cytokine, effectively regulating AMP synthesis, melanization response and cellular immunity to eliminate invading pathogens.

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Ostrinia furnacalis 生长阻断肽的多相免疫调节作用。

昆虫依靠先天性免疫系统消灭病原微生物。作为该系统的一个组成部分，细胞因子传递细胞间信号以控制免疫反应。生长抑素（GBP）是应激反应肽家族中的一种细胞因子，存在于包括果蝇在内的几种昆虫中。然而，人们对 GBP 在防御病原体方面的生理作用还不甚了解。在这项研究中，我们探讨了 GBP 在鳞翅目害虫 Ostrinia furnacalis 中的功能。注射重组 O. furnacalis GBP（OfGBP）前体（proGBP）和化学合成的 GBP 能显著诱导抗菌肽（AMPs）和其他免疫相关基因（包括免疫缺陷（IMD）和 Dorsal）的转录。细菌感染后，OfGBP mRNA 水平上调。敲除 OfGBP 的表达会导致 IMD、Relish、MyD88 和 Dorsal mRNA 水平下降。OfGBP 可诱导酚氧化酶活性，并影响 O. furnacalis 幼虫的血细胞行为。总之，GBP 是一种强效细胞因子，能有效调节 AMP 合成、黑色化反应和细胞免疫，从而消灭入侵的病原体。

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来源期刊

Insect Molecular Biology 生物-昆虫学

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).