An association between Dnmt1 and Wnt in the production of oocytes in the whitefly Bemisia tabaci

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2024-02-09 DOI:10.1111/imb.12893
Christopher B. Cunningham, Emily A. Shelby, Elizabeth C. McKinney, Alvin M. Simmons, Allen J. Moore, Patricia J. Moore
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Abstract

The function of DNA methylation in insects and the DNA methyltransferase (Dnmt) genes that influence methylation remains uncertain. We used RNA interference to reduce the gene expression of Dnmt1 within the whitefly Bemisia tabaci (Hemiptera:Aleyrodidae; Gennadius), a hemipteran species that relies on Dnmt1 for proper gametogenesis. We then used RNA-seq to test an a priori hypothesis that meiosis-related genetic pathways would be perturbed. We generally did not find an overall effect on meiosis-related pathways. However, we found that genes in the Wnt pathway, genes associated with the entry into meiosis in vertebrates, were differentially expressed. Our results are consistent with Dnmt1 knockdown influencing specific pathways and not causing general transcriptional response. This is a finding that is also seen with other insect species. We also characterised the methylome of B. tabaci and assessed the influence of Dnmt1 knockdown on cytosine methylation. This species has methylome characteristics comparable to other hemipterans regarding overall level, enrichment within gene bodies, and a bimodal distribution of methylated/non-methylated genes. Very little differential methylation was observed, and difference in methylation were not associated with differences in gene expression. The effect on Wnt presents an interesting new candidate pathway for future studies.

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Dnmt1 和 Wnt 与烟粉虱卵母细胞的产生有关。
昆虫 DNA 甲基化的功能以及影响甲基化的 DNA 甲基转移酶(Dnmt)基因仍不确定。我们利用 RNA 干扰减少了烟粉虱(Hemiptera:Aleyrodidae; Gennadius)体内 Dnmt1 的基因表达。然后,我们使用 RNA-seq 对减数分裂相关遗传通路会受到干扰这一先验假设进行了检验。一般来说,我们没有发现减数分裂相关通路受到整体影响。但我们发现,Wnt 通路中的基因,即与脊椎动物进入减数分裂相关的基因,出现了差异表达。我们的结果与 Dnmt1 基因敲除影响特定通路而非引起一般转录反应的结果一致。这一发现在其他昆虫物种中也可以看到。我们还描述了 B. tabaci 的甲基组特征,并评估了 Dnmt1 敲除对胞嘧啶甲基化的影响。在整体水平、基因体内的富集程度以及甲基化/非甲基化基因的双峰分布方面,该物种的甲基组特征与其他半翅目昆虫相当。观察到的甲基化差异很小,甲基化差异与基因表达差异无关。对 Wnt 的影响为今后的研究提供了一个有趣的新候选途径。
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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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