Detection of US and Kobe-type Babesia microti in ticks collected from small mammals of the Republic of Korea

IF 1.2 4区 农林科学 Q3 ENTOMOLOGY Entomological Research Pub Date : 2024-02-10 DOI:10.1111/1748-5967.12708
Tae Yun Kim, Seong Yoon Kim, Jiye Seo, Hee IL Lee, Wook-Gyo Lee, Hyunwoo Kim
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Abstract

Human babesiosis is an emerging tick-borne zoonotic disease prevalent in large parts of the world. This study aimed to examine the status of Babesia microti infection in ticks collected from small mammals captured during the nationwide survey of the Republic of Korea (ROK), 2017. Ixodid ticks from small mammals of three species, Apodemus agrarius, Crocidura lasiura, and Tscherskia triton, were used for the molecular detection of B. microti. PCR analyses amplifying 18S ribosomal RNA (rRNA) of the genus Babesia showed 8.4% of the ticks to be positive. Nucleotide sequencing of the PCR products revealed two different genotypes of B. microti, the US and Kobe-type. The US-types of B. microti were detected in ticks from small mammals of almost all study sites, whereas detections of Kobe-types were restricted to ticks from southern part of ROK. Phylogenetic analysis also indicated that each of the two types of B. microti clustered into two distinct clades. This is the first report on the presence of the US and Kobe-type B. microti in ticks collected from small mammals trapped on a national scale in ROK.

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从大韩民国小型哺乳动物身上采集的蜱虫中检测到美国型和科贝型微小巴贝斯菌
人类巴贝西亚原虫病是一种新出现的蜱媒人畜共患病,在世界大部分地区流行。本研究旨在考察 2017 年大韩民国(ROK)全国调查期间从捕获的小型哺乳动物身上采集的蜱虫中小肠巴贝西亚原虫的感染状况。研究人员利用来自三种小型哺乳动物(Apodemus agrarius、Crocidura lasiura和Tscherskia triton)的伊科蜱对微小巴贝西亚原虫进行了分子检测。扩增巴贝西亚属 18S 核糖体 RNA(rRNA)的 PCR 分析显示,8.4% 的蜱呈阳性。PCR 产物的核苷酸测序显示,小蜱属有两种不同的基因型,即 US 型和 Kobe 型。几乎所有研究地点的小型哺乳动物蜱虫中都检测到了 US 型微小弧菌,而检测到的 Kobe 型微小弧菌仅限于韩国南部的蜱虫。系统发育分析还表明,两种类型的 B. microti 分属两个不同的支系。这是首次报告在韩国全国范围内从捕获的小型哺乳动物身上采集的蜱虫中发现了美国型和科贝型微小蜱。
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来源期刊
CiteScore
2.50
自引率
7.70%
发文量
64
期刊介绍: Entomological Research is the successor of the Korean Journal of Entomology. Published by the Entomological Society of Korea (ESK) since 1970, it is the official English language journal of ESK, and publishes original research articles dealing with any aspect of entomology. Papers in any of the following fields will be considered: -systematics- ecology- physiology- biochemistry- pest control- embryology- genetics- cell and molecular biology- medical entomology- apiculture and sericulture. The Journal publishes research papers and invited reviews.
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