{"title":"SOX2 Overlapping Transcript (<i>SOX2-OT</i>) Enhances the Lung Cancer Malignancy Through Interaction with <i>miR-194-5p/</i>SOX5 Axis.","authors":"Zahra Sadeghi, Fatemeh Dodangeh, Jamshid Raheb","doi":"10.30498/ijb.2023.364919.3530","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Lung cancer is one of the most common types of cancer and a leading cause of cancer-related deaths worldwide. Therefore, it is useful to know the biomarkers involved in the malignancy of lung cancer.</p><p><strong>Objectives: </strong>This study aimed to show that <i>SOX2-OT</i> as a long non-coding RNA (IncRNA) regulates gene expression via the <i>SOX2-OT/miR-194-5p/</i>SOX5 axis molecular pathway in lung cancer.</p><p><strong>Materials and methods: </strong>A549 cells transfected with siRNA-<i>SOX2-OT</i> and the expression of <i>SOX2-OT</i> and <i>miR-194-5p</i> genes were analyzed by real-time PCR before and after transfection. In addition, the expression of the B-catenin, MMP9, phosphorylated and activated STAT3 (p-STAT3), SOX5, and VEGF proteins before and after transfection was investigated by Western blotting.</p><p><strong>Results: </strong>After using siRNA-<i>SOX2-OT</i>, an increase in the expression of <i>miR-194-5p</i> and a decrease in the expression of B-catenin, SOX5, p-STAT3 activated STAT3, VEGF, and MMP9 proteins was observed.</p><p><strong>Conclusions: </strong>According to the results of the present study, an increase in <i>SOX2-OT</i> in lung cancer seems to stimulate the expression of beta-catenin, SOX5, MMP9, and VEGF thus support the malignancy of lung cancer cells.</p>","PeriodicalId":14492,"journal":{"name":"Iranian Journal of Biotechnology","volume":"21 3","pages":"e3530"},"PeriodicalIF":1.6000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10858351/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.30498/ijb.2023.364919.3530","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
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Abstract
Background: Lung cancer is one of the most common types of cancer and a leading cause of cancer-related deaths worldwide. Therefore, it is useful to know the biomarkers involved in the malignancy of lung cancer.
Objectives: This study aimed to show that SOX2-OT as a long non-coding RNA (IncRNA) regulates gene expression via the SOX2-OT/miR-194-5p/SOX5 axis molecular pathway in lung cancer.
Materials and methods: A549 cells transfected with siRNA-SOX2-OT and the expression of SOX2-OT and miR-194-5p genes were analyzed by real-time PCR before and after transfection. In addition, the expression of the B-catenin, MMP9, phosphorylated and activated STAT3 (p-STAT3), SOX5, and VEGF proteins before and after transfection was investigated by Western blotting.
Results: After using siRNA-SOX2-OT, an increase in the expression of miR-194-5p and a decrease in the expression of B-catenin, SOX5, p-STAT3 activated STAT3, VEGF, and MMP9 proteins was observed.
Conclusions: According to the results of the present study, an increase in SOX2-OT in lung cancer seems to stimulate the expression of beta-catenin, SOX5, MMP9, and VEGF thus support the malignancy of lung cancer cells.
期刊介绍：
Iranian Journal of Biotechnology (IJB) is published quarterly by the National Institute of Genetic Engineering and Biotechnology. IJB publishes original scientific research papers in the broad area of Biotechnology such as, Agriculture, Animal and Marine Sciences, Basic Sciences, Bioinformatics, Biosafety and Bioethics, Environment, Industry and Mining and Medical Sciences.
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