Human papillomavirus type 16 E7 promotes cell viability and migration in cervical cancer by regulating the miR-23a/HOXC8 axis.

IF 0.9 4区医学 Q4 OBSTETRICS & GYNECOLOGY Journal of Obstetrics and Gynaecology Pub Date : 2024-12-01 Epub Date: 2024-02-13 DOI:10.1080/01443615.2024.2311658

Yahang Chen, Lei Sun, Lin Li

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Abstract

Background: Human papillomavirus (HPV) is a risk factor for the occurrence of cervical cancer (CC). Here, we aimed to explore the role of HPV16 in CC and identify the underlying mechanism.

Methods: The expression of miR-23a, HPV16 E6/E7 and homeobox C8 (HOXC8) was measured by quantitative real-time PCR or western blot. Cell viability and migration were evaluated using cell counting kit-8, Transwell and wound healing assays. The targeting relationship between miR-23a and HOXC8 was revealed by dual-luciferase reporter assay.

Results: miR-23a was downregulated in HPV16-positive (HPV16+) CC tissues and HPV16+ and HPV18+ cells. Additionally, E6/E7 expression was increased in CC cells. Then, we found that E7, rather than E6, positively regulated miR-23a expression. miR-23a suppressed cell viability and migration, whereas E7 overexpression abrogated this suppression. miR-23a targeted HOXC8, which reversed miR-23a-mediated cell viability and migration.

Conclusions: HPV16 E7-mediated miR-23a suppressed CC cell viability and migration by targeting HOXC8, suggesting a novel mechanism of HPV-induced CC.

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人乳头瘤病毒 16 型 E7 通过调控 miR-23a/HOXC8 轴促进宫颈癌细胞的活力和迁移。

背景：人乳头瘤病毒（HPV）是宫颈癌（CC）发生的危险因素之一。在此，我们旨在探索 HPV16 在宫颈癌中的作用并确定其潜在机制：方法：采用定量实时 PCR 或 Western 印迹法测定 miR-23a、HPV16 E6/E7 和同源染色体 C8（HOXC8）的表达。使用细胞计数试剂盒-8、Transwell 和伤口愈合试验评估细胞活力和迁移。结果显示：miR-23a在HPV16阳性（HPV16+）CC组织、HPV16+和HPV18+细胞中下调。此外，CC 细胞中 E6/E7 表达增加。miR-23a抑制了细胞的活力和迁移，而E7的过量表达则减弱了这种抑制作用：结论：HPV16 E7介导的miR-23a通过靶向HOXC8抑制了CC细胞的活力和迁移，提示了HPV诱导CC的新机制。

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来源期刊

Journal of Obstetrics and Gynaecology 医学-妇产科学

CiteScore

2.40

自引率

7.70%

发文量

398

审稿时长

6 months

期刊介绍： Journal of Obstetrics and Gynaecology represents an established forum for the entire field of obstetrics and gynaecology, publishing a broad range of original, peer-reviewed papers, from scientific and clinical research to reviews relevant to practice. It also includes occasional supplements on clinical symposia. The journal is read widely by trainees in our specialty and we acknowledge a major role in education in Obstetrics and Gynaecology. Past and present editors have recognized the difficulties that junior doctors encounter in achieving their first publications and spend time advising authors during their initial attempts at submission. The journal continues to attract a world-wide readership thanks to the emphasis on practical applicability and its excellent record of drawing on an international base of authors.