MicroRNA Signatures for Pancreatic Cancer and Chronic Pancreatitis: Expression Profiling by NGS.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-03-01 Epub Date: 2024-02-10 DOI:10.1097/MPA.0000000000002297

Nidhi Singh, Imteyaz Ahmad Khan, Safoora Rashid, Sumaira Rashid, Shikha Roy, Kriti Kaushik, Amit Kumar, Prasenjit Das, Sanjeev Lalwani, Dinesh Gupta, Deepak Gunjan, Nihar Ranjan Dash, Shyam S Chauhan, Surabhi Gupta, Anoop Saraya

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Abstract

Background: Pancreatic ductal adenocarcinoma (PDAC) is a deadly disease due to the lack of early detection. Because chronic pancreatitis (CP) patients are a high-risk group for pancreatic cancer, this study aimed to assess the differential miRNA profile in pancreatic tissue of patients with CP and pancreatic cancer.

Methods: MiRNAs were isolated from formalin-fixed paraffin-embedded pancreatic tissue of 22 PDAC patients, 18 CP patients, and 10 normal pancreatic tissues from autopsy (C) cases and processed for next-generation sequencing. Known and novel miRNAs were identified and analyzed for differential miRNA expression, target prediction, and pathway enrichment between groups.

Results: Among the miRNAs identified, 166 known and 17 novel miRNAs were found exclusively in PDAC tissues, while 106 known and 10 novel miRNAs were found specifically in CP tissues. The pathways targeted by PDAC-specific miRNAs and differentially expressed miRNAs between PDAC versus CP tissues and PDAC versus control tissues were the proteoglycans pathway, Hippo signaling pathway, adherens junction, and transforming growth factor-β signaling pathway.

Conclusions: This study resulted in a set of exclusive and differentially expressed miRNAs in PDAC and CP can be assessed for their diagnostic value. In addition, studying the role of miRNA-target gene interactions in carcinogenesis may open new therapeutic avenues.

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胰腺癌和慢性胰腺炎的 MicroRNA 标志：通过 NGS 进行表达谱分析。

背景：由于缺乏早期发现，胰腺导管腺癌（PDAC）是一种致命疾病。由于慢性胰腺炎（CP）患者是胰腺癌的高危人群，本研究旨在评估CP和胰腺癌患者胰腺组织中不同的miRNA谱：从福尔马林固定石蜡包埋的 22 例 PDAC 患者、18 例 CP 患者和 10 例尸检（C）正常胰腺组织中分离出 miRNA，并进行新一代测序。结果发现了已知的和新的 miRNAs，并对不同组间的 miRNA 表达差异、靶点预测和通路富集进行了分析：结果：在鉴定出的 miRNA 中，166 个已知 miRNA 和 17 个新型 miRNA 专门存在于 PDAC 组织中，而 106 个已知 miRNA 和 10 个新型 miRNA 专门存在于 CP 组织中。PDAC特异性miRNA靶向的通路以及PDAC相对于CP组织和PDAC相对于对照组织之间差异表达的miRNA是蛋白多糖通路、Hippo信号通路、粘连接头和转化生长因子-β信号通路：结论：这项研究发现了一组在 PDAC 和 CP 中独有且差异表达的 miRNA，可用于评估其诊断价值。此外，研究 miRNA 与靶基因的相互作用在癌变中的作用可能会开辟新的治疗途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.