Integration of IgM ELISA and 56 kDa gene PCR in management of pediatric acute encephalitis syndrome associated with scrub typhus

IF 2.9 4区医学 Q2 INFECTIOUS DISEASES Infectious diseases now Pub Date : 2024-02-11 DOI:10.1016/j.idnow.2024.104865

Pooja Bhardwaj , Vishal Yadav , Alok Sharma , Shahzadi Gulafshan , Sthita Pragnya Behera , Gaurav Raj Dwivedi , Hirawati Deval , Vijayachari Paluru , Manoj Murhekar , Rajeev Singh

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Abstract

Objectives

To identify the potential target genes for detection of Orientia tsutsugamushi (OT) in pediatric acute encephalitis syndrome (pAES).

Methods

DNA was extracted from whole blood of 100 pAES cases having tested positive (n = 41) and negative (n = 59) for scrub typhus (ST) by IgM ELISA. These samples were subjected to standard PCR for 56 kDa, 47 kDa, 16 s rRNA, groEL, traD genes and the newly identified 27 kDa gene.

Results

Among the selected gene targets, 56 kDa demonstrated its superiority for OT detection over the other tested genes. The presence of OT was confirmed via PCR targeting 56 kDa gene in 17 out of the 41 (41.4 %) IgM-positive ST AES cases and 38 out of the 59 (64.4 %) ST IgM negative cases. None of the other gene targets were amplified.

Conclusion

Integration of serological diagnosis with molecular diagnostics targeting the 56 kDa gene for routine testing of AES patients would facilitate detection of OT in AES endemic regions.

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将 IgM 酶联免疫吸附试验和 56 kDa 基因 PCR 纳入与恙虫病相关的小儿急性脑炎综合征的治疗中。

目的确定检测小儿急性脑炎综合征（pAES）中恙虫病（OT）的潜在靶基因：从 100 例经 IgM ELISA 检测为恙虫病（ST）阳性（41 例）和阴性（59 例）的小儿急性脑炎综合征病例的全血中提取 DNA。对这些样本进行了56 kDa、47 kDa、16s rRNA、groEL、traD基因和新发现的27 kDa基因的标准PCR检测：结果：在选定的目标基因中，56 kDa 基因在检测 OT 方面优于其他检测基因。在 41 例（41.4%）IgM 阳性的 ST AES 病例中，有 17 例通过以 56 kDa 基因为靶点的 PCR 结果证实了 OT 的存在；在 59 例（64.4%）IgM 阴性的 ST AES 病例中，有 38 例通过以 56 kDa 基因为靶点的 PCR 结果证实了 OT 的存在。其他目标基因均未扩增：结论：将血清学诊断与针对 56 kDa 基因的分子诊断相结合，用于 AES 患者的常规检测，将有助于在 AES 流行地区检测出 OT。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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