Elena S Ivanova, Maria A Belova, Olga Yu Rumiantseva, Alena A Zudilova, Dmitry S Kopylov, Michael Y Borisov, Viktor T Komov
{"title":"Effects of lipid extraction on stable isotope ratios of carbon and nitrogen in muscles of freshwater fish.","authors":"Elena S Ivanova, Maria A Belova, Olga Yu Rumiantseva, Alena A Zudilova, Dmitry S Kopylov, Michael Y Borisov, Viktor T Komov","doi":"10.1080/10256016.2024.2317379","DOIUrl":null,"url":null,"abstract":"<p><p>The extraction of lipids by the Folch method from the muscles of all the fish studied led to statistically significant differences in the values of <i>δ</i><sup>15</sup>N. At the same time, lipid extraction led to a statistically significant increase in <i>δ</i><sup>13</sup>C in pike and roach, and to a statistically insignificant decrease in <i>δ</i><sup>13</sup>C in perch and bream. Thus, lipid extraction cannot serve as a universal method of sample preparation for the analysis of the isotopic composition of carbon (<sup>13</sup>C/<sup>12</sup>C) and nitrogen (<sup>15</sup>N/<sup>14</sup>N) in fish muscles. The differences between the <i>δ</i><sup>13</sup>C values in the samples before and after lipid extraction were statistically investigated by different models. It is shown that mathematical correction method models can be used, but the results are depending on the fish types.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1080/10256016.2024.2317379","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/14 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
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Abstract
The extraction of lipids by the Folch method from the muscles of all the fish studied led to statistically significant differences in the values of δ15N. At the same time, lipid extraction led to a statistically significant increase in δ13C in pike and roach, and to a statistically insignificant decrease in δ13C in perch and bream. Thus, lipid extraction cannot serve as a universal method of sample preparation for the analysis of the isotopic composition of carbon (13C/12C) and nitrogen (15N/14N) in fish muscles. The differences between the δ13C values in the samples before and after lipid extraction were statistically investigated by different models. It is shown that mathematical correction method models can be used, but the results are depending on the fish types.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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