OVEREXPRESSION OF PTEN GENE INCREASES INS2 GENE MRNA EXPRESSION, NOT INS1 GENE MRNA EXPRESSION, IN INSULINOMA CELL LINE RIN-5F.

IF 0.7 4区 医学 Q4 ENDOCRINOLOGY & METABOLISM Acta Endocrinologica-Bucharest Pub Date : 2023-07-01 Epub Date: 2024-02-01 DOI:10.4183/aeb.2023.277
T Kiba
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Abstract

Objective: One functional neuroendocrine tumor that causes hypoglycemia due to inappropriately high insulin production is an insulinoma. In rats, two genes coding for insulin, insulin 1 (Ins1) and insulin 2 (Ins2) are found on chromosome 1. Ins1 was produced from an Ins2 transcript, and it was inserted into the genome via an RNA-mediated duplication-transposition event, according to some structural feature analyses.

Methods: In this study, the author has looked at how overexpression of the PTEN gene in the insulinoma cell line Rin-5F affects the expression of the insulin genes, Ins 1 and Ins 2.

Results: In the insulinoma cell line, overexpression of the PTEN gene boosts Ins2 gene mRNA expression but not Ins1 gene mRNA expression. It has been reported that PTEN upregulates insulin signaling by increasing insulin receptor substrate (IRS)-2 mRNA levels. Also, PTEN has been reported to be secreted in exosomes and thereafter, into extracellular space.

Conclusions: The present study suggested that overexpression of PTEN might induce the increasing Ins 2 gene expression, one of the phosphorylated genes against the IRS-2 through the insulin/IGF-1 receptor. Our knowledge of the molecular pathways of PTEN relating the synthesis of insulin has been increased by the present study.

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在胰岛素瘤细胞系 rin-5f 中,过表达 pten 基因会增加 ins2 基因 mrna 的表达,而不会增加 ins1 基因 mrna 的表达。
目的:胰岛素瘤是一种功能性神经内分泌肿瘤,会因胰岛素分泌过多而导致低血糖。大鼠的 1 号染色体上有两个编码胰岛素的基因,即胰岛素 1(Ins1)和胰岛素 2(Ins2)。Ins1由Ins2转录本产生,根据一些结构特征分析,它是通过RNA介导的复制-转座事件插入基因组的:在这项研究中,作者考察了胰岛素瘤细胞系 Rin-5F 中 PTEN 基因的过度表达如何影响胰岛素基因 Ins 1 和 Ins 2 的表达:结果:在胰岛素瘤细胞系中,PTEN基因的过表达会促进Ins2基因mRNA的表达,但不会促进Ins1基因mRNA的表达。据报道,PTEN 通过增加胰岛素受体底物(IRS)-2 mRNA 水平来上调胰岛素信号转导。此外,有报道称 PTEN 会在外泌体中分泌,然后进入细胞外空间:本研究表明,PTEN 的过表达可能会通过胰岛素/IGF-1 受体诱导 IRS-2 磷酸化基因之一的 Ins 2 基因表达增加。本研究增加了我们对 PTEN 与胰岛素合成相关的分子途径的了解。
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来源期刊
Acta Endocrinologica-Bucharest
Acta Endocrinologica-Bucharest 医学-内分泌学与代谢
CiteScore
1.30
自引率
20.00%
发文量
53
审稿时长
6-12 weeks
期刊介绍: Acta Endocrinologica (Buc) is an international journal covering the fields of basic and clinical Endocrinology, Neuroendocrinology, Reproductive Medicine, Chronobiology, Human Ethology published quarterly Acta Endocrinologica (Buc) is the official international journal of the Romanian Society for Endocrinology. It continues the former Romanian Journal of Endocrinology
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