Circulating tRNA-derived fragments are decreased in patients with rheumatoid arthritis and increased in patients with psoriatic arthritis.

IF 2 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biomarkers Pub Date : 2024-03-01 Epub Date: 2024-02-27 DOI:10.1080/1354750X.2024.2319297

Marina Dunaeva, Jan Blom, Rogier Thurlings, Margot van Weijsten, Fons A J van de Loo, Ger J M Pruijn

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Abstract

Introduction: tRNA-derived fragments (tRFs) play an important role in immune responses. To clarify the role of tRFs in autoimmunity we studied circulating tRF-levels in patients with rheumatoid arthritis (RA) and psoriatic arthritis (PsA), and in a murine model for arthritis.

Material and methods: Circulating tRF-levels were quantified by miR-Q RT-qPCR. tRNA processing and modification enzyme expression was analysed by RT-qPCR and public transcriptomics data.

Results: Significant reduction (up to 3-fold on average) of tRF-levels derived from tRNA-Gly-GCC,CCC, tRNA-Glu-CTC and tRNA-Val-CAC,AAC was observed in RA patients, whereas tRNA-Glu-CTC and tRNA-Val-CAC,AAC tRFs were found at significantly higher levels (up to 3-fold on average) in PsA patients, compared to healthy controls. Also in arthritic IL1Ra-KO mice reduced levels of tRNA-Glu-CTC fragments were seen. The expression of NSUN2, a methyltransferase catalysing tRNA methylation, was increased in RA-peripheral blood mononuclear cells (PBMCs) compared to PsA, but this is not consistently supported by public transcriptomics data.

Discussion: The observed changes of specific tRF-levels may be involved in the immune responses in RA and PsA and may be applicable as new biomarkers.

Conclusion: Circulating tRF-levels are decreased in RA and increased in PsA and this may, at least in part, be mediated by methylation changes.

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类风湿性关节炎患者体内循环 tRNA 衍生片段减少，而银屑病关节炎患者体内循环 tRNA 衍生片段增加。

导言 - tRNA 衍生片段（tRFs）在免疫反应中发挥着重要作用。为了明确 tRFs 在自身免疫中的作用，我们研究了类风湿性关节炎（RA）和银屑病关节炎（PsA）患者以及小鼠关节炎模型中的循环 tRF 水平。结果 - 与健康对照组相比，在 RA 患者中观察到来自 tRNA-Gly-GCC,CCC、tRNA-Glu-CTC 和 tRNA-Val-CAC,AAC 的 tRF 水平显著降低（平均高达 3 倍），而在 PsA 患者中发现 tRNA-Glu-CTC 和 tRNA-Val-CAC,AAC tRF 水平显著升高（平均高达 3 倍）。此外，在患有关节炎的 IL1Ra-KO 小鼠中，tRNA-Glu-CTC 片段的水平也有所降低。讨论 - 观察到的特定 tRF 水平的变化可能参与了 RA 和 PsA 的免疫反应，并可作为新的生物标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomarkers 医学-毒理学

CiteScore

5.00

自引率

3.80%

发文量

140

审稿时长

3 months

期刊介绍： The journal Biomarkers brings together all aspects of the rapidly growing field of biomarker research, encompassing their various uses and applications in one essential source. Biomarkers provides a vital forum for the exchange of ideas and concepts in all areas of biomarker research. High quality papers in four main areas are accepted and manuscripts describing novel biomarkers and their subsequent validation are especially encouraged: • Biomarkers of disease • Biomarkers of exposure • Biomarkers of response • Biomarkers of susceptibility Manuscripts can describe biomarkers measured in humans or other animals in vivo or in vitro. Biomarkers will consider publishing negative data from studies of biomarkers of susceptibility in human populations.