Loss of function of OsL1 gene cause early flowering in rice under short-day conditions

IF 2.6 3区农林科学 Q1 AGRONOMY Molecular Breeding Pub Date : 2024-02-16 DOI:10.1007/s11032-024-01444-1

{"title":"Loss of function of OsL1 gene cause early flowering in rice under short-day conditions","authors":"","doi":"10.1007/s11032-024-01444-1","DOIUrl":null,"url":null,"abstract":"<h3>Abstract </h3> Heading date is one of the important agronomic traits that affects rice yield. In this study, we cloned a new rice B3 family gene, OsL1, which regulates rice heading date. Importantly, osl1-1 and osl1-2, two different types of mutants of OsL1 were created using the gene editing technology CRISPR/Cas9 system and exhibited 4 days earlier heading date than that of the wild type under short-day conditions. Subsequently, the plants overexpressing OsL1, OE-OsL1, showed a 2-day later heading date than the wild type in Changsha and a 5-day later heading date in Lingshui, but there was no significant difference in other yield traits. Moreover, the results of subcellular localization study indicated that OsL1 protein was located in the nucleus and the expression pattern analysis showed that OsL1 gene was expressed in rice roots, stems, leaves, and panicles, and the expression level was higher at the root and weak green panicle. In addition, the OsL1 gene was mainly expressed at night time under short-light conditions. The transcriptomic analysis indicated that OsL1 might be involved in the Hd1-Hd3a pathway function. Together, our results revealed that the cloning and functional analysis of OsL1 can provide new strategy for molecular design breeding of rice with suitable fertility period.","PeriodicalId":18769,"journal":{"name":"Molecular Breeding","volume":"31 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Breeding","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11032-024-01444-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Heading date is one of the important agronomic traits that affects rice yield. In this study, we cloned a new rice B3 family gene, OsL1, which regulates rice heading date. Importantly, osl1-1 and osl1-2, two different types of mutants of OsL1 were created using the gene editing technology CRISPR/Cas9 system and exhibited 4 days earlier heading date than that of the wild type under short-day conditions. Subsequently, the plants overexpressing OsL1, OE-OsL1, showed a 2-day later heading date than the wild type in Changsha and a 5-day later heading date in Lingshui, but there was no significant difference in other yield traits. Moreover, the results of subcellular localization study indicated that OsL1 protein was located in the nucleus and the expression pattern analysis showed that OsL1 gene was expressed in rice roots, stems, leaves, and panicles, and the expression level was higher at the root and weak green panicle. In addition, the OsL1 gene was mainly expressed at night time under short-light conditions. The transcriptomic analysis indicated that OsL1 might be involved in the Hd1-Hd3a pathway function. Together, our results revealed that the cloning and functional analysis of OsL1 can provide new strategy for molecular design breeding of rice with suitable fertility period.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

OsL1 基因功能缺失导致水稻在短日照条件下提早开花

摘要发棵期是影响水稻产量的重要农艺性状之一。在这项研究中，我们克隆了一个新的水稻 B3 家族基因 OsL1，它能调控水稻的发棵期。重要的是，我们利用基因编辑技术CRISPR/Cas9系统创建了OsL1的两种不同突变体osl1-1和osl1-2，它们在短日照条件下比野生型早4天抽穗。随后，过表达 OsL1 的植株（OE-OsL1）在长沙的打顶期比野生型晚 2 天，在陵水的打顶期比野生型晚 5 天，但在其他产量性状上没有显著差异。此外，亚细胞定位研究结果表明，OsL1 蛋白位于细胞核中，表达模式分析表明，OsL1 基因在水稻根、茎、叶和圆锥花序中均有表达，且在根部和弱绿圆锥花序中表达水平较高。此外，在短光照条件下，OsL1 基因主要在夜间表达。转录组分析表明，OsL1可能参与了Hd1-Hd3a通路的功能。综上所述，我们的研究结果表明，OsL1基因的克隆和功能分析可为适宜生育期水稻的分子设计育种提供新策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.