Open-label, Multi-arm Randomized Clinical Trial Evaluating Vitamin E and Selenium Injection, Vitamin C Injection, and Hydrogen Peroxide Gavage as a Treatment for Gastric Cryptosporidiosis in Eastern Indigo Snakes (Drymarchon couperi)

James E. Bogan, Bethany Jackson, Michelle Hoffman, Michael M. Garner, April Childress, Nick Clark
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Abstract

Gastric cryptosporidiosis (GC) is an insidious infection in squamates caused by the protozoan Cryptosporidium serpentis and has impacted the captive breeding colony for the eastern indigo snake (Drymarchon couperi; EIS) reintroduction program. This study investigates a novel treatment of GC in EIS. Seventeen EIS with GC were randomly divided into three groups: A, B, and C. Group A (n = 6) received parenteral administration of 25 mg/kg vitamin C, 0.5 mg/kg vitamin E, and 50 μg/kg selenium and 5 ml/kg 3 % H2O2 gavage; Group B (n = 6) received the same injections but 5 ml/kg water gavage; and Group C received no treatments and served as controls. All EIS from Groups A and B tested negative for C. serpentis for three months following treatment, while only 60% (3/5) in Group C tested negative.  EIS testing negative received one 4 mg/kg dexamethasone sodium-phosphate injection. For three months following dexamethasone, 66.7% (4/6) in Group A continued to test negative compared to 83.3% (5/6) in Group B and 20% (1/5) in Group C. EIS testing negative underwent gastric biopsies but only one from Group C was confirmed to be negative for C. serpentis. Although parenteral vitamin C, vitamin E, and selenium with H2O2 gavage decreased shedding of C. serpentis, it did not outperform the vitamins/selenium without H2O2. The parenteral use of 25 mg/kg vitamin C, 0.5 mg/kg vitamin E, and 50 ug/kg selenium once weekly cannot be recommended for treatment of C. serpentis in EIS if complete resolution of the parasite is desired.
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评估维生素 E 和硒注射液、维生素 C 注射液以及过氧化氢灌胃法治疗东方蓝靛蛇(Drymarchon couperi)胃隐孢子虫病的开放标签、多臂随机临床试验
胃隐孢子虫病(GC)是一种由蛇形隐孢子虫原生动物引起的有鳞类动物隐性感染,已对东部靛蓝蛇(Drymarchon couperi; EIS)重引进计划的圈养繁殖地造成了影响。本研究调查了一种治疗 EIS GC 的新方法。17 条患有 GC 的 EIS 被随机分为三组:A 组(n = 6)接受 25 mg/kg 维生素 C、0.5 mg/kg 维生素 E 和 50 μg/kg 硒的肠外注射,以及 5 ml/kg 3 % H2O2 的灌胃;B 组(n = 6)接受相同的注射,但灌胃 5 ml/kg 水;C 组不接受任何治疗,作为对照。A 组和 B 组的所有 EIS 在治疗后三个月的蛇虫检测结果均为阴性,而 C 组只有 60% 的 EIS(3/5)检测结果为阴性。 检测结果为阴性的 EIS 接受了一次 4 毫克/千克的地塞米松磷酸钠注射。地塞米松注射三个月后,66.7%(4/6)的 A 组检测结果仍为阴性,而 B 组为 83.3%(5/6),C 组为 20%(1/5)。虽然肠外维生素 C、维生素 E 和硒配合 H2O2 灌胃可减少蛇尾畸形的脱落,但其效果并不优于不配合 H2O2 灌胃的维生素/硒。如果希望彻底清除寄生虫,则不能推荐每周一次肠外使用 25 毫克/千克维生素 C、0.5 毫克/千克维生素 E 和 50 微克/千克硒来治疗 EIS 中的蛇虫属寄生虫。
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