Bacterial load in meconium

IF 23.7 Q1 MICROBIOLOGY iMeta Pub Date : 2024-02-13 DOI:10.1002/imt2.173
Wen-Yu Jin, Jing Peng, Jinping Dai, Rongkang Tang, Jia-Xin Guo, Huan Zhao, Jielin Wang, Shu Zhang, Yi-Zhou Gao
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Abstract

The spike-in plasmid method was utilized to perform an analysis on meconium and second-pass feces, yielding both relative and absolute quantitative results. With the absolute quantitative data, the abundance of bacteria in 17 meconium samples and 17 second-pass fecal samples were found to be 1.14 × 107 and 1.59 × 109 copies/g, respectively. The mode of delivery can significantly influence the alterations and compositions of gut bacteria in a newborn within 72 h.

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胎粪中的细菌量
利用插入质粒法对胎粪和二次粪便进行分析,得出相对和绝对定量结果。绝对定量数据显示,17 份胎膜样本和 17 份二胎粪便样本中的细菌丰度分别为 1.14 × 107 和 1.59 × 109 拷贝/克。分娩方式会在 72 小时内对新生儿肠道细菌的变化和组成产生重大影响。
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