Low-Frequency Dynamic Magnetic Fields Decrease Cellular Uptake of Magnetic Nanoparticles

IF 4.7 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-02-01 DOI:10.3390/magnetochemistry10020009

A. Ivanova, Nelly S. Chmelyuk, A. Nikitin, Alexander G. Majouga, V. Chekhonin, M. Abakumov

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Abstract

Magnetic nanoparticles have gained attention as a potential structure for therapy and diagnosing oncological diseases. The key property of the magnetic nanoparticles is the ability to respond to an external magnetic field. It is known that magnetofection causes an increase in the cellular uptake of RNA and DNA in complexes with magnetic nanoparticles in the presence of a permanent magnetic field. However, the influence of a dynamic magnetic field on the internalization of MNPs is not clear. In this work, we propose the idea that applying external low-frequency dynamic magnetic fields may decrease the cellular uptake, such as macrophages and malignant neuroblastoma. Using fluorescence microscopy and atomic emission spectroscopy, we found that oscillating magnetic fields decreased the cellular uptake of magnetic nanoparticles compared to untreated cells by up to 46%. In SH-SY5Y tumor cells and macrophage RAW264.7 cells, the absolute values of Fe per cell differed by 0.10 pg/cell and 0.33 pg/cell between treated and untreated cells, respectively. These results can be applied in the control of the cellular uptake in different areas of biomedicine.

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低频动态磁场减少细胞对磁性纳米粒子的吸收

磁性纳米粒子作为一种治疗和诊断肿瘤疾病的潜在结构，已受到广泛关注。磁性纳米粒子的关键特性是对外部磁场的反应能力。众所周知，在永久磁场存在的情况下，磁感染会增加细胞对磁性纳米粒子复合物中 RNA 和 DNA 的吸收。然而，动态磁场对 MNPs 内化的影响尚不清楚。在这项工作中，我们提出了外部低频动态磁场可能会减少细胞摄取（如巨噬细胞和恶性神经母细胞瘤）的观点。我们利用荧光显微镜和原子发射光谱发现，与未经处理的细胞相比，振荡磁场可使细胞对磁性纳米粒子的吸收率降低达 46%。在 SH-SY5Y 肿瘤细胞和 RAW264.7 巨噬细胞中，处理过的细胞和未处理的细胞中每个细胞铁的绝对值分别相差 0.10 pg/cell 和 0.33 pg/cell。这些结果可用于控制生物医学不同领域的细胞吸收。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.