Refined metabolite profiling in the collateral circulation of chronic total occlusion of coronary arteries: Insights from a metabolomics investigation

IF 1.4 Q3 PERIPHERAL VASCULAR DISEASE Atherosclerosis plus Pub Date : 2024-02-12 DOI:10.1016/j.athplu.2024.02.001

Hu Sigan, Li Min, Cheng Zengwei, Gao Shiyi, Kang Pinfang, Gao Dasheng

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Abstract

Background and aims

To investigate the disparities in coronary collateral circulation (CCC) and peripheral serum metabolites among patients presenting with chronic total occlusion (CTO) of the coronary arteries, a non-targeted metabolic approach was employed.

Methods

A cohort of 22 patients diagnosed with CTO of coronary arteries in the context of coronary heart disease (CHD) was selected for blood sample collection from CCC and peripheral arteries. The patients were categorized into two groups, namely CTO-C and CTO-P. The Waters UPLC I-Class Plus is combined with the Q Exactive high-resolution mass spectrometer for metabolite separation and detection. The acquired raw data from mass spectrometry is subsequently imported into Compound Discoverer 3.2 software for comprehensive analysis, which seamlessly integrates the BGI Metabolome Database (BMDB), mzCloud database, and ChemSpider online database. Subsequently, the identified differential metabolites were subjected to a metabolic pathway enrichment analysis, as documented in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database.

Results

A total of 403 differential metabolites were identified in CCC and peripheral serum samples from patients with CTO of coronary arteries in CHD. Compared to the CTO-P group, the CTO-C group exhibited decreased levels of metabolites such as Testosterone, dehydroepiandrosterone (DHA), deoxyacetone, while demonstrating increased levels of metabolites including Progesterone, androstanone, l-threonine. The biosynthesis pathway of steroid hormones emerges as the key metabolic pathway significantly associated with differential metabolites.

Conclusions

Through metabolomics analysis, distinct differences in the CCC and peripheral serum metabolites have been identified among patients with CTO of coronary artery. Notably, a significant association between the steroid hormone biosynthesis pathway and CCC has been observed.

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慢性冠状动脉完全闭塞侧支循环的精细代谢物分析：代谢组学调查的启示

背景和目的为了研究冠状动脉慢性全闭塞（CTO）患者冠状动脉侧支循环（CCC）和外周血清代谢物的差异，我们采用了一种非靶向代谢方法。方法我们选取了22例被诊断为冠状动脉CTO的冠心病（CHD）患者，采集他们的CCC和外周动脉血样本。患者被分为两组，即 CTO-C 组和 CTO-P 组。沃特世 UPLC I-Class Plus 与 Q Exactive 高分辨率质谱仪相结合，用于代谢物的分离和检测。质谱获得的原始数据随后被导入 Compound Discoverer 3.2 软件进行综合分析，该软件无缝集成了 BGI 代谢组数据库（BMDB）、mzCloud 数据库和 ChemSpider 在线数据库。结果在冠状动脉CTO患者的CCC和外周血清样本中，共鉴定出403种差异代谢物。与CTO-P组相比，CTO-C组睾酮、脱氢表雄酮（DHA）、脱氧丙酮等代谢物水平降低，而黄体酮、雄甾酮、l-苏氨酸等代谢物水平升高。结论通过代谢组学分析，发现冠状动脉 CTO 患者的 CCC 和外周血清代谢物存在明显差异。值得注意的是，类固醇激素生物合成途径与 CCC 之间存在明显关联。

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