MicroRNA-153 Restrains Cell Metastasis and Epithelial–Mesenchymal Transition in Cervical Carcinoma by Targeting SATB1 and Regulating Wnt/β-Catenin Pathway
Wenfeng Liu, Meng Zhang, Xiaojie Du, Min Zhang, Weiling Wang, Zhiying Zhang
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引用次数: 0
Abstract
As a malignant tumor, cervical cancer (CC) seriously affects women’s life and health. Various microRNAs (miRNAs) are involved in tumorigenesis of CC. Here, we mainly paid attention to the effect of miR-153 in CC. RT-qPCR or Western blot was employed to quantify miR-153 or SATB1
expression. Molecular mechanism of miR-153/SATB1 was detected by Transwell and dual-luciferase assays. MiR-153 was downregulated in CC. Furthermore, upregulation of miR-153 restrained cell metastasis. Upregulation of SATB1 was detected in CC, and negative connected with miR-153 in CC cells.
Knockdown of SATB1 suppressed cell metastasis in CC. The inhibitory effect of miR-153 was abolished by upregulation of SATB1. Besides that, miR-153 blocked EMT and downregulated p-β-catenin expression in CC cells. MiR-153 restrains cell metastasis and EMT in CC by targeting SATB1
and regulating Wnt/β-catenin pathway.
宫颈癌（CC）作为一种恶性肿瘤，严重影响着女性的生活和健康。多种微RNA（miRNA）参与了CC的肿瘤发生。在此，我们主要关注 miR-153 在 CC 中的作用。采用RT-qPCR或Western blot定量检测miR-153或SATB1的表达。通过Transwell和双荧光素酶实验检测了miR-153/SATB1的分子机制。CC中的miR-153被下调。此外，miR-153 的上调抑制了细胞的转移。在 CC 中检测到 SATB1 的上调，并且在 CC 细胞中与 miR-153 呈负相关。敲除SATB1抑制了CC细胞的转移。SATB1的上调会取消miR-153的抑制作用。此外，miR-153还能阻止EMT，并下调CC细胞中p-β-catenin的表达。MiR-153通过靶向SATB1和调节Wnt/β-catenin通路抑制CC细胞的转移和EMT。
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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