Correlations of ADAMTS13 Expression with Kidney Injury and Inflammatory Cytokine Changes in Rats with Preeclampsia

Abstract

This study investigated the altered expression of ADAMTS13 (a metalloproteinase) in a rat model of preeclampsia (PE)-induced kidney injury, along with its connection to inflammatory cytokines. Sprague-Dawley rats were divided into PE and Control groups. PE group rats were induced with Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME) to simulate PE-induced kidney injury. Key indicators such as systolic and diastolic blood pressure and proteinuria were assessed to confirm PE model success. Kidney tissue changes were examined through histology and cell apoptosis, while ADAMTS13 expression was studied using Western blotting and qRT-PCR. Plasma ADAMTS13 levels were correlated with interleukin-6 (IL-6) and IL-8 cytokines in PE rats. Elevated SBP, DBP, and proteinuria were evident in L-NAME-treated pregnant rats. Microscopic examination revealed glomerulocystic changes, thickened basement membranes, and increased apoptotic cells. Compared to Controls, PE rats exhibited decreased ADAMTS13 expression in renal tissues and plasma. Plasma IL-6 and IL-8 levels were elevated, inversely proportional to ADAMTS13 concentration in PE rats. In conclusion, PE-induced kidney injury reduced ADAMTS13 levels, correlating with heightened IL-6 and IL-8 concentrations, suggesting an interplay between ADAMTS13 and inflammatory cytokines.