Investigating the therapeutic potential of Bifidobacterium breve and Lactobacillus rhamnosus postbiotics through apoptosis induction in colorectal HT-29 cancer cells

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-10 DOI:10.18502/ijm.v16i1.14873

Nafiseh Erfanian, Hossein Safarpour, Tahmineh Tavakoli, Mohammad Ali Mahdiabadi, S. Nasseri, Mohammad Hassan Namaei

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Abstract

Background and Objectives: Colorectal cancer (CRC) is a prevalent form of cancer worldwide. Recent studies suggest that postbiotics derived from probiotic bacteria have the potential as an adjunct therapy for CRC. This study investigates the anti-cancer effects of Bifidobacterium breve (B. breve) and Lactobacillus rhamnosus (L. rhamnosus) postbiotics on the HT-29 cell line. Materials and Methods: Through MTT and scratch assay, we investigated the anti-proliferation and anti-migration effects of B. breve and L. rhamnosus postbiotics on HT-29 cells. Furthermore, postbiotic-mediated apoptosis was assessed by ana- lyzing the expression of Bax, Bcl-2, and caspase-3. We also investigated the effects of B. breve postbiotics on the expression of three important genes involved in metastasis, including RSPO2, NGF, and MMP7. Consequently, we validated the expres- sion of selected genes in twelve adenocarcinoma tissues. Results: The results demonstrated the significant impact of postbiotics on HT-29 cells, highlighting their ability to induce anti-proliferation, anti-migration, and apoptosis-related effects. Notably, these effects were more pronounced using B. breve postbiotics than L. rhamnosus. Additionally, B. breve postbiotics could inhibit metastasis through upregulation of RSPO2 while downregulating NGF and MMP7 expression in HT-29 cells. Conclusion: Our research suggests that postbiotic metabolites may be effective biological products for the prevention and treatment of cancer.

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通过诱导结直肠 HT-29 癌细胞凋亡研究双歧杆菌和鼠李糖乳杆菌益生元的治疗潜力

背景和目的：结肠直肠癌（CRC）是全球流行的一种癌症。最近的研究表明，从益生菌中提取的益生元有可能成为 CRC 的辅助疗法。本研究调查了前列双歧杆菌（B. breve）和鼠李糖乳杆菌（L. rhamnosus）益生元对 HT-29 细胞系的抗癌作用。材料与方法：通过 MTT 和划痕试验，我们研究了布氏乳杆菌和鼠李糖乳杆菌益生菌对 HT-29 细胞的抗增殖和抗迁移作用。此外，我们还通过分析 Bax、Bcl-2 和 caspase-3 的表达评估了益生菌介导的细胞凋亡。我们还研究了布氏杆菌益生元对三个参与转移的重要基因（包括 RSPO2、NGF 和 MMP7）表达的影响。因此，我们在 12 个腺癌组织中验证了所选基因的表达。结果显示结果表明，益生元对 HT-29 细胞有显著影响，突出了它们诱导抗增殖、抗迁移和凋亡相关效应的能力。值得注意的是，与鼠李糖酵母相比，乳头酵母益生菌的这些作用更为明显。此外，布氏乳杆菌益生菌还能通过上调 RSPO2 抑制转移，同时下调 HT-29 细胞中 NGF 和 MMP7 的表达。结论我们的研究表明，益生菌后代谢产物可能是预防和治疗癌症的有效生物制品。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.