Comparison of virulence factors between ESBL and non-ESBL producing Klebsiella pneumoniae isolates

IF 1.3 Q4 MICROBIOLOGY Iranian Journal of Microbiology Pub Date : 2024-02-10 DOI:10.18502/ijm.v16i1.14869

Hamed Hatami Mirbag, Saeed Tajbakhsh, Azam Askari, F. Yousefi

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Abstract

Background and Objectives: Klebsiella pneumoniae is an opportunistic pathogen responsible for causing nosocomial and community-acquired infections. Its pathogenicity is associated with a variety of virulence factors and antibiotic resistance. The aim of the present study was to compare virulence attributes between ESBL and non-ESBL producing isolates. Materials and Methods: A total of 113 K. pneumoniae including 56 ESBL and 57 non ESBL-producers were collected in Bushehr province, Iran, from November 2017 to February 2019. Enzymatic profile, hypermucoviscosity and biofilm forma- tion were investigated phenotypically. In addition, the presence of rmpA, aerobactin, kfu, allS, mrkD, ybtS, entB, iutA, fimH, wabG, wcaG, K1 and K2 genes were detected by PCR and sequencing. Results: There was no statistically significant difference in enzymatic profile between ESBL and non-ESBL producers. The prevalence of the hypermocoviscosity was lower among ESBL compared to non-ESBL producers but the intensity of biofilm was higher in the ESBL producers. Among the virulence genes, K1, rmpA, iutA, and aero were observed only in non-ESBLs. Moreover, the carriage of allS, K, K2, rmpA, iutA and aero genes was higher in hypermucoviscous in comparison with non hypermucoviscous isolates. Conclusion: The identification of potentially pathogenic isolates plays an important role in preventing their spread as well as the success of their treatment.

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ESBL与不产生ESBL的肺炎克雷伯菌分离物毒力因子的比较

背景和目的：肺炎克雷伯氏菌是一种机会性病原体，可引起院内感染和社区获得性感染。其致病性与多种毒力因子和抗生素耐药性有关。本研究旨在比较产生 ESBL 和不产生 ESBL 的分离株的毒力属性。材料与方法：2017年11月至2019年2月，在伊朗布什尔省共收集了113株肺炎克氏菌，包括56株产ESBL和57株非产ESBL菌。从表型上调查了酶谱、高黏度和生物膜形成情况。此外，还通过 PCR 和测序检测了 rmpA、aerobactin、kfu、allS、mrkD、ybtS、entB、iutA、fimH、wabG、wcaG、K1 和 K2 基因的存在。结果ESBL和非ESBL生产者的酶谱差异无统计学意义。与非 ESBL 生产者相比，ESBL 生产者的高黏度发生率较低，但 ESBL 生产者的生物膜强度较高。在毒力基因中，仅在非 ESBLs 中观察到 K1、rmpA、iutA 和 aero。此外，与非高黏液性分离物相比，高黏液性分离物携带的 allS、K、K2、rmpA、iutA 和 aero 基因较多。结论识别潜在的致病分离物对防止其传播和成功治疗起着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.