Effects of Dietary Phytol Supplementation on Growth Performance, Immunological Parameters, Intestinal Bacteria, and Prevention of Oxidative Stress Following Transportation of Nile Tilapia, Oreochromis niloticus

IF 3 2区农林科学 Q1 FISHERIES Aquaculture Nutrition Pub Date : 2024-02-07 DOI:10.1155/2024/7039179

Saman Ahani, Sara Ahani, Morteza Yousefi, Ali Taheri Mirghaed, Afaf N. Abdel Rahman

{"title":"Effects of Dietary Phytol Supplementation on Growth Performance, Immunological Parameters, Intestinal Bacteria, and Prevention of Oxidative Stress Following Transportation of Nile Tilapia, Oreochromis niloticus","authors":"Saman Ahani, Sara Ahani, Morteza Yousefi, Ali Taheri Mirghaed, Afaf N. Abdel Rahman","doi":"10.1155/2024/7039179","DOIUrl":null,"url":null,"abstract":"Nile tilapia, Oreochromis niloticus, (2.00 ± 0.02 g) were reared in 16 70-L tanks (40 individual/tank) and fed diets (approx. 345 g/kg protein, approx. 87 g/kg crude fat) containing 0 (CTL), 75 (PH-75), 150 (PH-150), and 300 (PH-300) mg/kg phytol (n = 4). After 60 days of feeding (4% daily), growth performance, humoral immune parameters, and gut bacteria were analyzed. Also, hepatic antioxidant parameters were determined before and after the fish were transported in plastic bags for 6 hr. The results showed that PH-75 exhibited the highest final weight (P < 0.001), weight gain (P < 0.001), feed intake (P < 0.001), feed efficiency (P = 0.015), plasma lysozyme activity (P = 0.004), and intestinal Lactobacillus sp. population (P = 0.017), among the treatments. The highest plasma alternative complement activity (P = 0.006) and the lowest intestinal total viable bacteria (P = 0.027) were observed in PH-75 and PH-150. The highest plasma alkaline phosphatase activities were observed in PH-75 and PH-300 (P = 0.014). The highest blood leukocyte (P = 0.008), monocyte (P = 0.010), and eosinophil (P < 0.001) were observed in PH-300, while the highest blood neutrophil was observed in all phytol treatments (P < 0.001). The highest hepatic lipid peroxidation was observed in PH-300, whereas PH-75 and PH-150 showed the lowest values (P < 0.001). The highest hepatic reduced glutathione was observed in PH-75, also PH-150 exhibited significant elevation in this parameter, compared to CTL (P < 0.001). Transportation led to significant elevations in the hepatic antioxidant enzymes’ activities in CTL, PH-75, and PH-150; the highest activities were related to PH-75 and PH-150 treatments, which had also the highest post-transportation survivals (P < 0.001). In conclusion, phytol is a suitable feed supplement for Nile tilapia, improving growth performance and welfare, particularly at 75 mg/kg.","PeriodicalId":8225,"journal":{"name":"Aquaculture Nutrition","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Nutrition","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/7039179","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Nile tilapia, Oreochromis niloticus, (2.00 ± 0.02 g) were reared in 16 70-L tanks (40 individual/tank) and fed diets (approx. 345 g/kg protein, approx. 87 g/kg crude fat) containing 0 (CTL), 75 (PH-75), 150 (PH-150), and 300 (PH-300) mg/kg phytol (n = 4). After 60 days of feeding (4% daily), growth performance, humoral immune parameters, and gut bacteria were analyzed. Also, hepatic antioxidant parameters were determined before and after the fish were transported in plastic bags for 6 hr. The results showed that PH-75 exhibited the highest final weight (P < 0.001), weight gain (P < 0.001), feed intake (P < 0.001), feed efficiency (P = 0.015), plasma lysozyme activity (P = 0.004), and intestinal Lactobacillus sp. population (P = 0.017), among the treatments. The highest plasma alternative complement activity (P = 0.006) and the lowest intestinal total viable bacteria (P = 0.027) were observed in PH-75 and PH-150. The highest plasma alkaline phosphatase activities were observed in PH-75 and PH-300 (P = 0.014). The highest blood leukocyte (P = 0.008), monocyte (P = 0.010), and eosinophil (P < 0.001) were observed in PH-300, while the highest blood neutrophil was observed in all phytol treatments (P < 0.001). The highest hepatic lipid peroxidation was observed in PH-300, whereas PH-75 and PH-150 showed the lowest values (P < 0.001). The highest hepatic reduced glutathione was observed in PH-75, also PH-150 exhibited significant elevation in this parameter, compared to CTL (P < 0.001). Transportation led to significant elevations in the hepatic antioxidant enzymes’ activities in CTL, PH-75, and PH-150; the highest activities were related to PH-75 and PH-150 treatments, which had also the highest post-transportation survivals (P < 0.001). In conclusion, phytol is a suitable feed supplement for Nile tilapia, improving growth performance and welfare, particularly at 75 mg/kg.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

膳食中补充植物醇对尼罗罗非鱼（Oreochromis niloticus）运输后的生长性能、免疫参数、肠道细菌和氧化应激预防的影响

将尼罗罗非鱼（2.00 ± 0.02 克）饲养在 16 个 70 升的水槽中（每槽 40 尾），喂食含 0 (CTL)、75 (PH-75)、150 (PH-150) 和 300 (PH-300) mg/kg 植醇的日粮（蛋白质约 345 克/千克，粗脂肪约 87 克/千克）（n = 4）。饲喂 60 天后（每天 4%），对生长性能、体液免疫参数和肠道细菌进行分析。此外，还测定了鱼在塑料袋中运输 6 小时前后的肝脏抗氧化参数。结果表明，在各处理中，PH-75的最终体重（P<0.001）、增重（P<0.001）、摄食量（P<0.001）、饲料效率（P=0.015）、血浆溶菌酶活性（P=0.004）和肠道乳酸杆菌数量（P=0.017）最高。PH-75 和 PH-150 的血浆替代补体活性最高（P=0.006），肠道总存活细菌数最低（P=0.027）。PH-75 和 PH-300 的血浆碱性磷酸酶活性最高（P=0.014）。在 PH-300 中观察到最高的血液白细胞（P=0.008）、单核细胞（P=0.010）和嗜酸性粒细胞（P<0.001），而在所有植醇处理中观察到最高的血液中性粒细胞（P<0.001）。PH-300 的肝脏脂质过氧化程度最高，而 PH-75 和 PH-150 的肝脏脂质过氧化程度最低（P<0.001）。与 CTL 相比，PH-75 的肝还原型谷胱甘肽最高，PH-150 的肝还原型谷胱甘肽也显著升高（P<0.001）。运输导致 CTL、PH-75 和 PH-150 的肝脏抗氧化酶活性明显升高；PH-75 和 PH-150 处理的活性最高，运输后存活率也最高（P<0.001）。总之，植醇是一种适用于尼罗罗非鱼的饲料添加剂，可改善生长性能和福利，尤其是在 75 毫克/千克的条件下。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Aquaculture Nutrition 农林科学-渔业

CiteScore

7.20

自引率

8.60%

发文量

131

审稿时长

3 months

期刊介绍： Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.