Identification of Aspergillus westerdijkiae and its potential risk of Ochratoxin A synthesis in Cannabis inflorescences

IF 1 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Scientia Agropecuaria Pub Date : 2024-02-05 DOI:10.17268/sci.agropecu.2024.004
Emily Aguirre-Ortega, Mónica Henao-López, Jolián A. Vargas-Alzate, Katherin Castro Ríos, C. N. Montoya-Estrada
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Abstract

Nowadays, fungal contamination of medical Cannabis inflorescences during postharvest has become an increasingly frequent and worrisome problem for consumers and the industry in general. This is because some of these microorganisms can produce secondary metabolites, such as mycotoxins, which can be toxic to humans. To assess the risk posed by fungal contamination and evaluate the potential for fungal isolates to produce mycotoxins, samples of medicinal Cannabis were tested for the presence of mycotoxin-forming fungi. Inflorescences were isolated on PDA agar at 23 ± 2 °C for ten days, and the microorganisms were identified. The strain with morphological characteristics compatible with the genus Aspergillus spp. was selected as the fungus with the highest risk of forming hazardous mycotoxins. This isolate was characterized conventionally and by molecular identification using primers for the internal transcribed spacer region (ITS) of ribosomal DNA and different coding genes and was identified as Aspergillus westerdijkiae. To determine mycotoxin formation, the genome of A. westerdijkiae was sequenced using the Illumina Novaseq platform in South Korea. The antiSMASH tool was used to search for gene clusters associated with producing secondary metabolites, and genes related to toxins were manually curated. Regions where the cluster of genes directly involved in OTA biosynthesis (otaA, otaB, otaC, otaR and otaD) were found, suggesting a potential risk of synthesis of this toxin.
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鉴定西红柿曲霉及其在大麻花序中合成赭曲霉毒素 A 的潜在风险
如今,医用大麻花序在收获后受到真菌污染的问题越来越频繁,也越来越令消费者和整个行业担忧。这是因为其中一些微生物会产生次生代谢物,如霉菌毒素,对人体有毒。为了评估真菌污染带来的风险,并评价真菌分离物产生霉菌毒素的可能性,对药用大麻样本进行了霉菌毒素形成真菌的检测。在 23±2 °C 的条件下,在 PDA 琼脂上分离花序十天,并对微生物进行鉴定。选出形态特征符合曲霉属的菌株,作为产生有害霉菌毒素风险最高的真菌。利用核糖体 DNA 内部转录间隔区(ITS)引物和不同的编码基因对这一分离菌株进行了常规鉴定和分子鉴定,确定其为西地岛曲霉。为了确定霉菌毒素的形成,在韩国使用 Illumina Novaseq 平台对 Westerdijkiae 的基因组进行了测序。使用 antiSMASH 工具搜索与产生次生代谢物有关的基因簇,并对与毒素有关的基因进行了人工整理。发现了直接参与 OTA 生物合成的基因簇(otaA、otaB、otaC、otaR 和 otaD)所在区域,表明存在合成这种毒素的潜在风险。
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来源期刊
Scientia Agropecuaria
Scientia Agropecuaria AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
3.50
自引率
0.00%
发文量
27
审稿时长
12 weeks
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