Erzsébet Varga, Noémi Birtalan, Abdulnabi Abushita, Ibolya Fülöp
{"title":"The Effects of Different Solvents on Phenolic, Flavonoid, Anthocyanin Contents and Free Radical Scavenging Activity on Pomegranate Juice and Seeds","authors":"Erzsébet Varga, Noémi Birtalan, Abdulnabi Abushita, Ibolya Fülöp","doi":"10.3311/ppch.21901","DOIUrl":null,"url":null,"abstract":"Our phytochemical investigation defined the polyphenol, anthocyanin and flavonoid content from methanolic, ethanolic 50% and watery extracts from fresh pomegranate juice and seeds with a spectrophotometric method. The antioxidant capacity was defined with the ABTS and DPPH methods. We determined the polyphenols with the Folin-Ciocalteu method, the anthocyanins with the method in Hungarian Pharmacopoeia VIIIth edition at Myrtilli fructus monography and the flavonoids with a modified method of the Romanian Pharmacopeia Xth edition Cynarae folium monography. We expressed the concentration of polyphenols in gallic acids (GA). The values indicated the following: in fruit juice, 47.57 μg GA/ml, in methanol seed extract 7 μg GA/g, the ethanol 50% and in watery seed extracts 8 μg GA/g. The definition of flavonoids indicated the following values expressed in quercetin (QE): in fruit juice 706 μg QE/ml, in methanol seed extract 416 μg QE/g, in ethanol 50% seed extract 46 μg QE/g and in watery seed extract 57 μg QE/g. The anthocyans from juice 3.95 mg/100 ml and seed 6.47 mg/100 g expressed in cyanidin-3-0-glycoside. We determined the following values with the ABTS method: the antioxidant capacity of juice is 25.40 μg/ml, in methanol seed extract 2.87 μg/ml, in ethanol seed extract 2.52 μg/ml, in watery 9.15 μg/ml. With the DPPH method, we obtained the following values: from juice 54.2 μg/ml, from methanolic extract 14.7 μg/ml, from ethanolic extract 10.58 μg/ml and watery extract 18.22 μg/ml. The received data shows the importance of pomegranate, which can be a new phytotherapeutic potential resource.","PeriodicalId":19922,"journal":{"name":"Periodica Polytechnica Chemical Engineering","volume":null,"pages":null},"PeriodicalIF":1.4000,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Periodica Polytechnica Chemical Engineering","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.3311/ppch.21901","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Our phytochemical investigation defined the polyphenol, anthocyanin and flavonoid content from methanolic, ethanolic 50% and watery extracts from fresh pomegranate juice and seeds with a spectrophotometric method. The antioxidant capacity was defined with the ABTS and DPPH methods. We determined the polyphenols with the Folin-Ciocalteu method, the anthocyanins with the method in Hungarian Pharmacopoeia VIIIth edition at Myrtilli fructus monography and the flavonoids with a modified method of the Romanian Pharmacopeia Xth edition Cynarae folium monography. We expressed the concentration of polyphenols in gallic acids (GA). The values indicated the following: in fruit juice, 47.57 μg GA/ml, in methanol seed extract 7 μg GA/g, the ethanol 50% and in watery seed extracts 8 μg GA/g. The definition of flavonoids indicated the following values expressed in quercetin (QE): in fruit juice 706 μg QE/ml, in methanol seed extract 416 μg QE/g, in ethanol 50% seed extract 46 μg QE/g and in watery seed extract 57 μg QE/g. The anthocyans from juice 3.95 mg/100 ml and seed 6.47 mg/100 g expressed in cyanidin-3-0-glycoside. We determined the following values with the ABTS method: the antioxidant capacity of juice is 25.40 μg/ml, in methanol seed extract 2.87 μg/ml, in ethanol seed extract 2.52 μg/ml, in watery 9.15 μg/ml. With the DPPH method, we obtained the following values: from juice 54.2 μg/ml, from methanolic extract 14.7 μg/ml, from ethanolic extract 10.58 μg/ml and watery extract 18.22 μg/ml. The received data shows the importance of pomegranate, which can be a new phytotherapeutic potential resource.
期刊介绍:
The main scope of the journal is to publish original research articles in the wide field of chemical engineering including environmental and bioengineering.