Yang Zhang, Jianglun Shen, Ning Li, Fen Hu, Faming Tian, Yiming Yang, Jinyin Yan, Haifeng Cai
{"title":"Mechanism of miR-21 Lipid Nanoparticles Carrier in Restraining Biological Behavior in Breast Carcinoma Through Targeting of Wnt/β-Catenin Channel","authors":"Yang Zhang, Jianglun Shen, Ning Li, Fen Hu, Faming Tian, Yiming Yang, Jinyin Yan, Haifeng Cai","doi":"10.1166/jbn.2024.3779","DOIUrl":null,"url":null,"abstract":"This study assessed the mechanism of miR-21 with lipid nanoparticles carrier in restraining biological behavior of breast carcinoma cells through targeting of Wnt/β-catenin channel. Breast carcinoma cells were collected and divided into blank set, miR-21 set, agonist set\n and inhibitor set. We observed expressions of miR-21 cyclinD1, Bcl-2, Bax and Caspases-3. Also, quantity of cells through basement membrane, expression of factors related with Wnt/β-catenin signal channel, and targeting correlation between miR-21 and Wnt were also observed. The\n expression of miR-21 in MCF-7 cells was lowest, while the ratio of active cells in blank set was highest. The expressions of Bax and Caspase-3 and quantity of cells through basement membrane in the blank and agonist sets were highest. The expressions of cyclinD1 and Bcl-2 were lowest. The\n apoptotic rate in the blank and agonist sets was lowest and invasive rate was highest. The expressions of Wnt and β-catenin in the blank and agonist sets were highest. There was direct targeting correlation between miR-21 and Wnt while Wnt/β-catenin activity was restrained\n by miR-21. The expressions of Bax and Caspase-3 also increased and apoptosis was induced and invasion and proliferation of breast carcinoma cells were restrained.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3779","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
This study assessed the mechanism of miR-21 with lipid nanoparticles carrier in restraining biological behavior of breast carcinoma cells through targeting of Wnt/β-catenin channel. Breast carcinoma cells were collected and divided into blank set, miR-21 set, agonist set
and inhibitor set. We observed expressions of miR-21 cyclinD1, Bcl-2, Bax and Caspases-3. Also, quantity of cells through basement membrane, expression of factors related with Wnt/β-catenin signal channel, and targeting correlation between miR-21 and Wnt were also observed. The
expression of miR-21 in MCF-7 cells was lowest, while the ratio of active cells in blank set was highest. The expressions of Bax and Caspase-3 and quantity of cells through basement membrane in the blank and agonist sets were highest. The expressions of cyclinD1 and Bcl-2 were lowest. The
apoptotic rate in the blank and agonist sets was lowest and invasive rate was highest. The expressions of Wnt and β-catenin in the blank and agonist sets were highest. There was direct targeting correlation between miR-21 and Wnt while Wnt/β-catenin activity was restrained
by miR-21. The expressions of Bax and Caspase-3 also increased and apoptosis was induced and invasion and proliferation of breast carcinoma cells were restrained.