A Convenient Ratiometric Fluorescent Probe Based on Gold Nanoclusters and Carbon Dots for Escherichia coli Determination

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food Analytical Methods Pub Date : 2024-02-17 DOI:10.1007/s12161-024-02595-9

Yongjie Liu, Jiayu Wang, Sunan Liu, Jing Li, Qian Xiang, Zaiyue Yang, Ling Zhu

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Abstract

Escherichia coli, as a prevalent foodborne pathogen, can harm people health seriously. Hence, developing the effective methods for E. coli determination is vital. In the paper, a convenient hybridization ratiometric fluorescent probe was constructed for the prompt determination of E. coli based on red emissive gold nanoclusters (AuNCs) and blue carbon dots (bCDs). The relevant experimental conditions were systematically optimized including copper concentration, incubation temperature, and time, in order to obtain the optimal results. There existed a strong linear relationship between the fluorescence intensity ratios (I₆₃₀/I₄₅₀) and the concentrations of E. coli for both AuNCs and bCDs. Based on the determination, it was found that the linear concentration range spanned from 10³ to 10⁷ CFU mL⁻¹, with a low limit of detection (LOD) of 120 CFU mL⁻¹. This method based on the mechanism of copper cocatalysis presents a sensitive and convenient strategy for E. coli determination, and has a promising future in the field of food safety.

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一种基于金纳米团簇和碳点的方便比率荧光探针，可用于大肠杆菌检测

大肠杆菌作为一种普遍存在的食源性病原体，会严重危害人们的健康。因此，开发有效的大肠杆菌检测方法至关重要。本文以红色发射型纳米金簇（AuNCs）和蓝色碳点（bCDs）为基础，构建了一种方便的杂交比率荧光探针，用于大肠杆菌的快速检测。为了获得最佳结果，对相关实验条件进行了系统优化，包括铜浓度、培养温度和时间。AuNCs 和 bCDs 的荧光强度比（I630/I450）与大肠杆菌浓度之间存在很强的线性关系。测定结果表明，线性浓度范围为 103-107 CFU mL-1，检测限（LOD）为 120 CFU mL-1。这种基于铜催化机理的方法为大肠杆菌的检测提供了一种灵敏、便捷的策略，在食品安全领域具有广阔的应用前景。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.