Neglected function of gastrin to reduce feeding in Siberian sturgeon (Acipenser baerii) via cholecystokinin receptor B.

IF 2.5 3区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Fish Physiology and Biochemistry Pub Date : 2024-06-01 Epub Date: 2024-02-21 DOI:10.1007/s10695-024-01308-7
Shupeng Zhang, Yongpei Lu, Yanling Liu, Mei Wang, Shaoqi Xu, Ya Li, Hongwei Wu, Qaolin Pei, Lei Yang, Lu Lu, Yixiao Xiong, Youlian Liu, Shuhuang Chen, Qin Yao, Qin Kang, Yingzi Li, Defang Chen, Xin Zhang, Ni Tang, Zhiqiong Li
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Abstract

Gastrin is an important intragastrointestinal hormone, but reports on its regulation of feeding behavior in fish are still scarce. This study aimed to determine the feeding regulatory function of gastrin in sturgeon. In this study, a gastrin/cholecystokinin-like peptide was identified in the genomes of sturgeon and proved to be gastrin by evolutionary tree analysis. Tissue distribution of gastrin and its receptor, cholecystokinin receptor B (CCKRB), showed that both had high mRNA abundance in the hypothalamus and gastrointestinal tract. In the duodenum, gastrin and CCKRB mRNAs were reduced at 1 h of fasting, and both were also observed in the stomach and hypothalamus in response to changes in feeding status. Sulfated gastrin 17 is the major form of gastrin in vivo. Therefore, we investigated the effect of sulfated gastrin 17 on feeding by intraperitoneal injection into Siberian sturgeon using sulfated gastrin 17. The results showed that gastrin 17 significantly reduced the cumulative feeding of Siberian sturgeon in the short term (1, 3 and 6 h) and long term (1, 2, 3, 4, 5 and 7 days). Finally, we explored the potential mechanism of feeding inhibition after intraperitoneal injection of gastrin 17 for 7 consecutive days. The results showed that gastrin 17 treatment significantly increased the mRNA levels of anorexigenic peptides (cart, cck and pyy), while it had no significant effect on the mRNA abundance of orexigenic peptides (npy and agrp). In addition, gastrin 17 treatment significantly affected the expression of appetite signaling pathways in the hypothalamus, such that the mRNA expression of ampkα1 was significantly reduced, whereas the mRNA abundance of stat3, mtor and s6k was significantly increased. In conclusion, the present study confirmed the anorectic effect of gastrin on Siberian sturgeon.

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被忽视的胃泌素通过胆囊收缩素受体 B 减少西伯利亚鲟(Acipenser baerii)摄食的功能。
胃泌素是一种重要的胃肠道激素,但有关它对鱼类摄食行为的调控作用的报道仍然很少。本研究旨在确定胃泌素在鲟鱼体内的摄食调节功能。该研究在鲟鱼基因组中发现了一种胃泌素/胆囊收缩素样肽,并通过进化树分析证明其为胃泌素。胃泌素及其受体胆囊收缩素受体B(CCKRB)在组织中的分布表明,两者在下丘脑和胃肠道中都有较高的mRNA丰度。在十二指肠中,空腹 1 小时后胃泌素和 CCKRB mRNA 会减少,在胃和下丘脑中也能观察到两者对进食状态变化的反应。硫酸化胃泌素 17 是体内胃泌素的主要形式。因此,我们通过对西伯利亚鲟腹腔注射硫酸化胃泌素17,研究了硫酸化胃泌素17对摄食的影响。结果表明,硫酸化胃泌素17在短期(1、3和6小时)和长期(1、2、3、4、5和7天)均能显著降低西伯利亚鲟的累积摄食量。最后,我们探讨了连续7天腹腔注射胃泌素17抑制摄食的潜在机制。结果显示,胃泌素17能显著提高厌食肽(cart、ck和pyy)的mRNA水平,而对厌食肽(npy和agrp)的mRNA丰度无明显影响。此外,胃泌素17还显著影响了下丘脑食欲信号通路的表达,如ampkα1的mRNA表达显著降低,而stat3、mtor和s6k的mRNA丰度则显著升高。总之,本研究证实了胃泌素对西伯利亚鲟的厌食作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Fish Physiology and Biochemistry
Fish Physiology and Biochemistry 农林科学-生化与分子生物学
CiteScore
5.60
自引率
6.90%
发文量
106
审稿时长
4 months
期刊介绍: Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.
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