In vitro micropropagation of a novel non-flowering radiation mutant of Platanus × acerifolia Willd.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-02-20 DOI:10.1007/s11627-023-10409-6

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Abstract

An efficient in vitro micropropagation system for Platanus × acerifolia ‘F03’, a radiation mutant that has not flowered for 19 yr, has been established. Axillary buds were used as explants. After sprouting on Murashige and Skoog medium (MS) supplemented with 0.3 mg·L⁻¹ 6-benzylaminopurine (6-BA) together with 0.05 mg·L⁻¹ α-naphthaleneacetic acid (NAA), the best medium for multiplication and elongation was MS basal medium supplemented with 0.1 mg·L⁻¹ 6-benzylaminopurine and with a multiplication rate of 3.43 ± 0.57. Plantlets over 1.5 cm could root on McCown Woody Plant Medium (WPM) supplemented with 0.01 mg·L⁻¹ α-naphthaleneacetic acid and 7.0 g·L⁻¹ agar with an ideal rooting rate and transplanting survival rate of 100 ± 0%. The optimum survival rate was possible when trays were covered with a thin plastic lid for more than 14 d. After transplanting, air humidity and temperature were crucial for young plant survival. The system established here could serve as a pathway for commercial production of the line and provide sterile leaf explants for regeneration and further genetic improvement of the species.

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一种新型非开花辐射突变体 Platanus × acerifolia Willd.

摘要为19年未开花的辐射突变体Platanus × acerifolia 'F03'建立了一个高效的离体微繁殖系统。腋芽被用作外植体。在添加了 0.3 mg-L-1 6-苄基氨基嘌呤（6-BA）和 0.05 mg-L-1 α-萘乙酸（NAA）的 Murashige 和 Skoog 培养基（MS）上萌发后，繁殖和伸长效果最好的培养基是添加了 0.1 mg-L-1 6-苄基氨基嘌呤的 MS 基础培养基，繁殖率为 3.43 ± 0.57。在添加了 0.01 mg-L-1 α-萘乙酸和 7.0 g-L-1 琼脂的麦考恩木本植物培养基（WPM）上，1.5 厘米以上的小苗可以生根，生根率和移栽成活率均为 100 ± 0%。移栽后，空气湿度和温度对幼苗的存活至关重要。这里建立的系统可作为该品系商业化生产的途径，并为该物种的再生和进一步遗传改良提供无菌叶片外植体。

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