Nescient helix-loop-helix 1 (Nhlh1) is a novel activating transcription factor 5 (ATF5) target gene in olfactory and vomeronasal sensory neurons in mice.

IF 3.2 3区 生物学 Q3 CELL BIOLOGY Cell and Tissue Research Pub Date : 2024-04-01 Epub Date: 2024-02-23 DOI:10.1007/s00441-024-03871-0
Chiharu Ishii, Haruo Nakano, Riko Higashiseto, Yusaku Ooki, Mariko Umemura, Shigeru Takahashi, Yuji Takahashi
{"title":"Nescient helix-loop-helix 1 (Nhlh1) is a novel activating transcription factor 5 (ATF5) target gene in olfactory and vomeronasal sensory neurons in mice.","authors":"Chiharu Ishii, Haruo Nakano, Riko Higashiseto, Yusaku Ooki, Mariko Umemura, Shigeru Takahashi, Yuji Takahashi","doi":"10.1007/s00441-024-03871-0","DOIUrl":null,"url":null,"abstract":"<p><p>Activating transcription factor 5 (ATF5) is a transcription factor that belongs to the cAMP-response element-binding protein/ATF family and is essential for the differentiation and survival of sensory neurons in mouse olfactory organs. However, transcriptional target genes for ATF5 have yet to be identified. In the present study, chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) experiments were performed to verify ATF5 target genes in the main olfactory epithelium and vomeronasal organ in the postnatal pups. ChIP-qPCR was conducted using hemagglutinin (HA)-tagged ATF5 knock-in olfactory organs. The results obtained demonstrated that ATF5-HA fusion proteins bound to the CCAAT/enhancer-binding protein-ATF response element (CARE) site in the enhancer region of nescient helix-loop-helix 1 (Nhlh1), a transcription factor expressed in differentiating olfactory and vomeronasal sensory neurons. Nhlh1 mRNA expression was downregulated in ATF5-deficient (ATF5<sup>-/-</sup>) olfactory organs. The LIM/homeobox protein transcription factor Lhx2 co-localized with ATF5 in the nuclei of olfactory and vomeronasal sensory neurons and bound to the homeodomain site proximal to the CARE site in the Nhlh1 gene. The CARE region of the Nhlh1 gene was enriched by the active enhancer marker, acetyl-histone H3 (Lys27). The present study identified Nhlh1 as a novel target gene for ATF5 in murine olfactory organs. ATF5 may upregulate Nhlh1 expression in concert with Lhx2, thereby promoting the differentiation of olfactory and vomeronasal sensory neurons.</p>","PeriodicalId":9712,"journal":{"name":"Cell and Tissue Research","volume":" ","pages":"85-94"},"PeriodicalIF":3.2000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell and Tissue Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00441-024-03871-0","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/23 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Activating transcription factor 5 (ATF5) is a transcription factor that belongs to the cAMP-response element-binding protein/ATF family and is essential for the differentiation and survival of sensory neurons in mouse olfactory organs. However, transcriptional target genes for ATF5 have yet to be identified. In the present study, chromatin immunoprecipitation-quantitative polymerase chain reaction (ChIP-qPCR) experiments were performed to verify ATF5 target genes in the main olfactory epithelium and vomeronasal organ in the postnatal pups. ChIP-qPCR was conducted using hemagglutinin (HA)-tagged ATF5 knock-in olfactory organs. The results obtained demonstrated that ATF5-HA fusion proteins bound to the CCAAT/enhancer-binding protein-ATF response element (CARE) site in the enhancer region of nescient helix-loop-helix 1 (Nhlh1), a transcription factor expressed in differentiating olfactory and vomeronasal sensory neurons. Nhlh1 mRNA expression was downregulated in ATF5-deficient (ATF5-/-) olfactory organs. The LIM/homeobox protein transcription factor Lhx2 co-localized with ATF5 in the nuclei of olfactory and vomeronasal sensory neurons and bound to the homeodomain site proximal to the CARE site in the Nhlh1 gene. The CARE region of the Nhlh1 gene was enriched by the active enhancer marker, acetyl-histone H3 (Lys27). The present study identified Nhlh1 as a novel target gene for ATF5 in murine olfactory organs. ATF5 may upregulate Nhlh1 expression in concert with Lhx2, thereby promoting the differentiation of olfactory and vomeronasal sensory neurons.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Nescient helix-loop-helix 1(Nhlh1)是小鼠嗅觉和绒毛感觉神经元中的一种新型激活转录因子 5(ATF5)靶基因。
激活转录因子5(ATF5)是一种转录因子,属于cAMP反应元件结合蛋白/ATF家族,对小鼠嗅觉器官感觉神经元的分化和存活至关重要。然而,ATF5的转录靶基因尚未确定。本研究通过染色质免疫沉淀-定量聚合酶链反应(ChIP-qPCR)实验来验证出生后幼鼠主嗅上皮细胞和绒毛器官中的 ATF5 靶基因。利用血凝素(HA)标记的 ATF5 基因敲入嗅觉器官进行了 ChIP-qPCR 试验。研究结果表明,ATF5-HA融合蛋白与Nescient helix-loop-helix 1(Nhlh1)增强子区域的CCAAT/增强子结合蛋白-ATF反应元件(CARE)位点结合。在 ATF5 缺失(ATF5-/-)的嗅觉器官中,Nhlh1 mRNA 表达下调。LIM/homeobox蛋白转录因子Lhx2与ATF5共同定位在嗅觉神经元和触角感觉神经元的细胞核中,并与Nhlh1基因中CARE位点近端的homeodomain位点结合。Nhlh1基因的CARE区域富含活性增强子标记乙酰组蛋白H3(Lys27)。本研究发现 Nhlh1 是 ATF5 在小鼠嗅觉器官中的一个新靶基因。ATF5可能与Lhx2共同上调Nhlh1的表达,从而促进嗅觉和绒毛感觉神经元的分化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Cell and Tissue Research
Cell and Tissue Research 生物-细胞生物学
CiteScore
7.00
自引率
2.80%
发文量
142
审稿时长
1 months
期刊介绍: The journal publishes regular articles and reviews in the areas of molecular, cell, and supracellular biology. In particular, the journal intends to provide a forum for publishing data that analyze the supracellular, integrative actions of gene products and their impact on the formation of tissue structure and function. Submission of papers with an emphasis on structure-function relationships as revealed by recombinant molecular technologies is especially encouraged. Areas of research with a long-standing tradition of publishing in Cell & Tissue Research include: - neurobiology - neuroendocrinology - endocrinology - reproductive biology - skeletal and immune systems - development - stem cells - muscle biology.
期刊最新文献
Single cell transcriptomics profiling of the stromal cells in the pathologic association of ribosomal proteins in the ischemic myocardium and epicardial fat. Expanding on roles of pleckstrin homology-like domain family A member 1 protein. Exploring the contribution of Zfp521/ZNF521 on primary hematopoietic stem/progenitor cells and leukemia progression. Mechanical stimulation promotes the maturation of cardiomyocyte-like cells from P19 cells and the function in a mouse model of myocardial infarction. Olfactory and gustatory chemical sensor systems in the African turquoise killifish: Insights from morphology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1