Towards a rational approach to promoter engineering: understanding the complexity of transcription initiation in prokaryotes.

IF 10.1 2区 生物学 Q1 MICROBIOLOGY FEMS microbiology reviews Pub Date : 2024-03-01 DOI:10.1093/femsre/fuae004
Cara Deal, Lien De Wannemaeker, Marjan De Mey
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Abstract

Promoter sequences are important genetic control elements. Through their interaction with RNA polymerase they determine transcription strength and specificity, thereby regulating the first step in gene expression. Consequently, they can be targeted as elements to control predictability and tuneability of a genetic circuit, which is essential in applications such as the development of robust microbial cell factories. This review considers the promoter elements implicated in the three stages of transcription initiation, detailing the complex interplay of sequence-specific interactions that are involved, and highlighting that DNA sequence features beyond the core promoter elements work in a combinatorial manner to determine transcriptional strength. In particular, we emphasize that, aside from promoter recognition, transcription initiation is also defined by the kinetics of open complex formation and promoter escape, which are also known to be highly sequence specific. Significantly, we focus on how insights into these interactions can be manipulated to lay the foundation for a more rational approach to promoter engineering.

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启动子工程的合理方法:了解原核生物转录启动的复杂性。
启动子序列是重要的基因控制元件。它们通过与 RNA 聚合酶的相互作用决定转录强度和特异性,从而调节基因表达的第一步。因此,启动子序列可以作为控制遗传回路可预测性和可调控性的目标元件,这在开发强大的微生物细胞工厂等应用中至关重要。本综述探讨了与转录启动三个阶段有关的启动子元件,详细介绍了其中涉及的序列特异性相互作用的复杂相互作用,并强调了核心启动子元件以外的 DNA 序列特征以组合方式决定转录强度。我们特别强调,除了启动子识别之外,转录启动还取决于开放复合物形成和启动子逸出的动力学,众所周知,这也具有高度的序列特异性。重要的是,我们将重点关注如何利用对这些相互作用的了解,为更合理的启动子工程学方法奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FEMS microbiology reviews
FEMS microbiology reviews 生物-微生物学
CiteScore
17.50
自引率
0.90%
发文量
45
审稿时长
6-12 weeks
期刊介绍: Title: FEMS Microbiology Reviews Journal Focus: Publishes reviews covering all aspects of microbiology not recently surveyed Reviews topics of current interest Provides comprehensive, critical, and authoritative coverage Offers new perspectives and critical, detailed discussions of significant trends May contain speculative and selective elements Aimed at both specialists and general readers Reviews should be framed within the context of general microbiology and biology Submission Criteria: Manuscripts should not be unevaluated compilations of literature Lectures delivered at symposia must review the related field to be acceptable
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