Turnip mosaic virus NIb weakens the function of eukaryotic translation initiation factor 6 facilitating viral infection in Nicotiana benthamiana.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-02-01 DOI:10.1111/mpp.13434
Ziqiang Chen, Feng Wang, Binghua Chen, Guanwei Wu, Dagang Tian, Quan Yuan, Shiyou Qiu, Yushan Zhai, Jianping Chen, Hongying Zheng, Fei Yan
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Abstract

Viruses rely completely on host translational machinery to produce the proteins encoded by their genes. Controlling translation initiation is important for gaining translational advantage in conflicts between the host and virus. The eukaryotic translation initiation factor 4E (eIF4E) has been reported to be hijacked by potyviruses for virus multiplication. The role of translation regulation in defence and anti-defence between plants and viruses is not well understood. We report that the transcript level of eIF6 was markedly increased in turnip mosaic virus (TuMV)-infected Nicotiana benthamiana. TuMV infection was impaired by overexpression of N. benthamiana eIF6 (NbeIF6) either transiently expressed in leaves or stably expressed in transgenic plants. Polysome profile assays showed that overexpression of NbeIF6 caused the accumulation of 40S and 60S ribosomal subunits, the reduction of polysomes, and also compromised TuMV UTR-mediated translation, indicating a defence role for upregulated NbeIF6 during TuMV infection. However, the polysome profile in TuMV-infected leaves was not identical to that in leaves overexpressing NbeIF6. Further analysis showed that TuMV NIb protein, the RNA-dependent RNA polymerase, interacted with NbeIF6 and interfered with its effect on the ribosomal subunits, suggesting that NIb might have a counterdefence role. The results propose a possible regulatory mechanism at the translation level during plant-virus interaction.

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芜菁花叶病毒 NIb 可削弱真核翻译起始因子 6 的功能,从而促进烟草花叶病毒的感染。
病毒完全依赖宿主的翻译机制来产生其基因编码的蛋白质。在宿主与病毒的冲突中,控制翻译起始对于获得翻译优势非常重要。据报道,真核翻译起始因子 4E(eIF4E)被壶状病毒劫持,用于病毒繁殖。翻译调控在植物与病毒之间的防御和反防御中的作用尚不十分清楚。我们报告说,在受芜菁花叶病毒(TuMV)感染的烟草中,eIF6 的转录水平显著增加。在叶片中瞬时表达或在转基因植物中稳定表达的 N. benthamiana eIF6(NbeIF6)的过表达抑制了 TuMV 感染。多聚体图谱分析表明,过表达 NbeIF6 会导致 40S 和 60S 核糖体亚基的积累、多聚体的减少,还会影响 TuMV UTR 介导的翻译,这表明上调的 NbeIF6 在 TuMV 感染过程中起着防御作用。然而,TuMV 感染叶片的多聚体特征与过表达 NbeIF6 的叶片并不相同。进一步分析表明,TuMV NIb 蛋白(RNA 依赖性 RNA 聚合酶)与 NbeIF6 相互作用,干扰了 NbeIF6 对核糖体亚基的影响,这表明 NIb 可能具有反防御作用。研究结果提出了植物与病毒相互作用过程中可能存在的翻译水平调控机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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