Assessment of circulating apoE4 levels from dried blood spot samples in a large survey setting.

IF 4 Q1 CLINICAL NEUROLOGY Alzheimer''s and Dementia: Diagnosis, Assessment and Disease Monitoring Pub Date : 2024-02-22 eCollection Date: 2024-01-01 DOI:10.1002/dad2.12555

Nis Borbye-Lorenzen, Yacila I Deza-Lougovski, Solveig Holmgaard, Luzia M Weiss, Marie Bækvad-Hansen, Kristin Skogstrand, Anna Rieckmann, Axel Börsch-Supan, Martina Börsch-Supan

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Abstract

Introduction: The apolipoprotein E (APOE) ε4 allele is associated with high risk for Alzheimer's disease. It is unclear whether individual levels of the circulating apoE4 protein in ε4 carriers confer additional risk. Measuring apoE4 protein levels from dried blood spots (DBS) has the potential to provide information on genetic status as well as circulating levels and to include these measures in large survey settings.

Methods: We developed a multiplex immunoassay to detect apoE4 protein levels in DBS from 15,974 participants, aged 50+ from Wave 6 of the Survey of Health, Ageing and Retirement in Europe (SHARE).

Results: The apoE4 protein signal was presented in two separable distributions. One distribution corresponded to carriers of at least one copy of the ε4 allele. Fieldwork cofounders affected protein levels but did not explain individual differences.

Discussion: Future research should investigate how genotype and apoE4 level interact with lifestyle and other variables to impact cognitive aging.

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在大规模调查中评估干血斑样本中的循环载脂蛋白 E4 水平。

简介载脂蛋白 E（APOE）ε4 等位基因与阿尔茨海默病的高风险有关。目前还不清楚ε4携带者循环中载脂蛋白E4蛋白的个体水平是否会带来额外风险。从干血斑（DBS）中测量载脂蛋白E4蛋白水平有可能提供有关遗传状态和循环水平的信息，并将这些测量方法纳入大型调查中：我们开发了一种多重免疫测定法，用于检测欧洲健康、老龄和退休调查（SHARE）第 6 波中 15974 名 50 岁以上参与者干血斑中的载脂蛋白 4 蛋白水平：结果：载脂蛋白E4蛋白信号呈两种可分离的分布。一种分布对应于至少一个ε4等位基因拷贝的携带者。现场工作的共同创始人会影响蛋白质水平，但不能解释个体差异：未来的研究应探讨基因型和载脂蛋白E4水平如何与生活方式和其他变量相互作用，从而影响认知老化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Alzheimer''s and Dementia: Diagnosis, Assessment and Disease Monitoring Medicine-Psychiatry and Mental Health

CiteScore

7.80

自引率

7.50%

发文量

101

审稿时长

8 weeks

期刊介绍： Alzheimer''s & Dementia: Diagnosis, Assessment & Disease Monitoring (DADM) is an open access, peer-reviewed, journal from the Alzheimer''s Association® that will publish new research that reports the discovery, development and validation of instruments, technologies, algorithms, and innovative processes. Papers will cover a range of topics interested in the early and accurate detection of individuals with memory complaints and/or among asymptomatic individuals at elevated risk for various forms of memory disorders. The expectation for published papers will be to translate fundamental knowledge about the neurobiology of the disease into practical reports that describe both the conceptual and methodological aspects of the submitted scientific inquiry. Published topics will explore the development of biomarkers, surrogate markers, and conceptual/methodological challenges. Publication priority will be given to papers that 1) describe putative surrogate markers that accurately track disease progression, 2) biomarkers that fulfill international regulatory requirements, 3) reports from large, well-characterized population-based cohorts that comprise the heterogeneity and diversity of asymptomatic individuals and 4) algorithmic development that considers multi-marker arrays (e.g., integrated-omics, genetics, biofluids, imaging, etc.) and advanced computational analytics and technologies.