Investigation of Reproductive Failure in Buffaloes at a Farm of Itarsi Tehsil in Madhya Pradesh in India for Detection of Antibodies to Brucella Species Organisms by Rose Bengal Test and Infectious Bovine Rhinotracheitis Virus by Indirect Enzyme-linked Immunosorbent Assay

Pub Date : 2024-02-17 DOI:10.18805/ijar.b-5233

S. Audarya, D. Chhabra, S. Nandi, R. Sharda, R. Gangil

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Abstract

Background: Reproductive failures due to abortion in buffaloes are a major cause of concern to livestock owners that cause economic losses. Brucellosis (bacterial disease) and infectious bovine rhinotracheitis (viral disease), both infectious diseases in buffaloes are implicated in abortions in India. Methods: The investigated buffalo farm experienced a few cases of abortions in pregnant animals in the late trimester. Blood samples were collected aseptically from individual animals without anticoagulants. After clotting of the blood, extracted serum samples were clarified and tested in Rose Bengal test and indirect enzyme-linked immunosorbent assay for the serological diagnosis of brucellosis and infectious bovine rhinotracheitis. Result: Out of 147 buffalo serum samples tested by using the Rose Bengal test, a total of 27 buffalo serum samples (18.36%) tested positive for the presence of agglutinating antibodies against Brucella species. None of the tested serum samples had detectable levels of antibodies against the infectious bovine rhinotracheitis virus in an indirect enzyme-linked immunosorbent assay.

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印度中央邦 Itarsi Tehsil 一家农场水牛繁殖失败调查，用玫瑰孟加拉试验检测布鲁氏菌抗体，用间接酶联免疫吸附试验检测传染性牛鼻气管炎病毒

背景：水牛流产导致的繁殖失败是牲畜所有者关注的一个主要问题，会造成经济损失。在印度，水牛流产与布鲁氏菌病（细菌性疾病）和传染性牛鼻气管炎（病毒性疾病）这两种传染性疾病有关。方法：被调查的水牛养殖场在妊娠晚期发生过几例流产。在不使用抗凝剂的情况下，无菌采集每头水牛的血液样本。血液凝固后，对提取的血清样本进行澄清，并通过玫瑰红试验和间接酶联免疫吸附试验对布氏杆菌病和传染性牛鼻气管炎进行血清学诊断。结果在使用玫瑰红试验检测的 147 份水牛血清样本中，共有 27 份水牛血清样本（18.36%）的布鲁氏菌凝集抗体呈阳性。在间接酶联免疫吸附试验中，检测的血清样本均未检测到牛传染性鼻气管炎病毒抗体。

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