Impact of Larval Food Source on the Stability of the Bactrocera dorsalis Microbiome

IF 3.3 3区生物学 Q2 ECOLOGY Microbial Ecology Pub Date : 2024-02-26 DOI:10.1007/s00248-024-02352-9

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Abstract

Bacterial symbionts are crucial to the biology of Bactrocera dorsalis. With larval diet (fruit host) being a key factor that determines microbiome composition and with B. dorsalis using more than 400 fruits as hosts, it is unclear if certain bacterial symbionts are preserved and are passed on to B. dorsalis progenies despite changes in larval diet. Here, we conducted a fly rearing experiment to characterize diet-induced changes in the microbiome of female B. dorsalis. In order to explicitly investigate the impacts of larval diet on the microbiome, including potential stable bacterial constituents of B. dorsalis, we performed 16S rRNA sequencing on the gut tissues of teneral female flies reared from four different host fruits (guava, mango, papaya, and rose apple) infested using a single cohort of wild B. dorsalis that emerged from tropical almond (mother flies). Although B. dorsalis-associated microbiota were predominantly shaped by the larval diet, some major bacterial species from the mother flies were retained in progenies raised on different larval diets. With some variation, Klebsiella (ASV 1 and 2), Morganella (ASV 3), and Providencia (ASV 6) were the major bacterial symbionts that were stable and made up 0.1–80% of the gut and ovipositor microbiome of female teneral flies reared on different host fruits. Our results suggest that certain groups of bacteria are stably associated with female B. dorsalis across larval diets. These findings provide a basis for unexplored research on symbiotic bacterial function in B. dorsalis and may aid in the development of novel management techniques against this devastating pest of horticultural importance.

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幼虫食物来源对多棘胸蛙微生物组稳定性的影响

摘要细菌共生体对背甲蛙的生物学至关重要。由于幼虫的饮食（水果寄主）是决定微生物组组成的关键因素，而且背翅蝇使用了 400 多种水果作为寄主，因此，尽管幼虫的饮食发生了变化，但某些细菌共生体是否会保存下来并传给背翅蝇的后代还不清楚。在这里，我们进行了一项苍蝇饲养实验，以描述饮食引起的雌性多角体微生物组变化的特征。为了明确研究幼虫饮食对微生物组的影响，包括背壳线虫潜在的稳定细菌成分，我们对从四种不同的寄主水果（番石榴、芒果、木瓜和玫瑰苹果）中饲养的一般雌蝇的肠道组织进行了 16S rRNA 测序，这些寄主水果使用了从热带杏仁中孳生的野生背壳线虫（母蝇）。虽然与背喙蝇相关的微生物群主要由幼虫食物决定，但在以不同幼虫食物饲养的后代中保留了来自母蝇的一些主要细菌种类。克雷伯氏菌（ASV 1 和 2）、摩根氏菌（ASV 3）和普罗维登西亚菌（ASV 6）是在不同寄主果实上饲养的雌性一般蝇肠道和产卵器微生物组中稳定的主要细菌共生体，占 0.1-80%。我们的研究结果表明，某些细菌群在不同的幼虫食谱中与雌性背翅蝇有稳定的联系。这些发现为研究背纹夜蛾体内共生细菌的功能提供了一个基础，并有助于开发新的管理技术来对付这种具有园艺重要性的毁灭性害虫。

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来源期刊

Microbial Ecology 生物-海洋与淡水生物学

CiteScore

6.90

自引率

2.80%

发文量

212

审稿时长

3-8 weeks

期刊介绍： The journal Microbial Ecology was founded more than 50 years ago by Dr. Ralph Mitchell, Gordon McKay Professor of Applied Biology at Harvard University in Cambridge, MA. The journal has evolved to become a premier location for the presentation of manuscripts that represent advances in the field of microbial ecology. The journal has become a dedicated international forum for the presentation of high-quality scientific investigations of how microorganisms interact with their environment, with each other and with their hosts. Microbial Ecology offers articles of original research in full paper and note formats, as well as brief reviews and topical position papers.