{"title":"<i>hsa_circ_0087100/hsa-miR-6743-5p</i> affects Th1 cell differentiation by regulating <i>STAT1</i> in diabetic retinopathy.","authors":"Shuai He, Dongwei Lai, Chenggong Ma, Chunren Meng, Chunyang Cai, Qian Chen, Chufeng Gu, Qinghua Qiu","doi":"10.2217/epi-2023-0359","DOIUrl":null,"url":null,"abstract":"<p><p><b>Objective:</b> To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). <b>Methods:</b> We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, <i>STAT1</i>-mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. <b>Results:</b> <i>hsa_circ_0087100/hsa-miR-6743-5p/STAT1</i> was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and <i>STAT1</i>-mediated Th1 differentiation was thus verified <i>in vitro</i> and <i>in vivo</i>. <b>Conclusion:</b> <i>hsa_circ_0087100/hsa-miR-6743-5p/STAT1</i> may affect Th1 cell differentiation in DR.</p>","PeriodicalId":11959,"journal":{"name":"Epigenomics","volume":" ","pages":"427-444"},"PeriodicalIF":3.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Epigenomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2217/epi-2023-0359","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/2/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To elucidate the role of the competitive endogenous RNA (ceRNA) network in immune infiltration of diabetic retinopathy (DR). Methods: We obtained differentially expressed (DE) circRNAs, miRNAs and mRNAs from the Gene Expression Omnibus database. Then, we identified immune infiltration by CIBERSORT and single-sample gene set enrichment analysis and discovered co-expression genes by weighted gene co-expression network analysis. Furthermore, STAT1-mediated Th1 differentiation was determined in DR cell models, DR patients and DR mouse models. Results:hsa_circ_0087100/hsa-miR-6743-5p/STAT1 was involved in immune infiltration of Th1 cells. Aberrant expression of the ceRNA network and STAT1-mediated Th1 differentiation was thus verified in vitro and in vivo. Conclusion:hsa_circ_0087100/hsa-miR-6743-5p/STAT1 may affect Th1 cell differentiation in DR.
期刊介绍：
Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community.
Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.
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