TGFβ2 mediates oxidative stress-induced epithelial-to-mesenchymal transition of bladder smooth muscle.

IF 1.5 4区 生物学 Q4 CELL BIOLOGY In Vitro Cellular & Developmental Biology. Animal Pub Date : 2024-08-01 Epub Date: 2024-02-26 DOI:10.1007/s11626-024-00864-9
Jingwen Geng, Xiaofan Zhang, Yansong Zhang, Xiaojia Meng, Jinqi Sun, Bo Zhou, Jun Ma
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Abstract

Bladder outlet obstruction (BOO) is the primary clinical manifestation of benign prostatic hyperplasia, the most common urinary system disease in elderly men, and leads to associated lower urinary tract symptoms. Although BOO is reportedly associated with increased systemic oxidative stress (OS), the underlying mechanism remains unclear. The elucidation of this mechanism is the primary aim of this study. A Sprague-Dawley rat model of BOO was constructed and used for urodynamic monitoring. The bladder tissue of rats was collected and subjected to real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR), histological examination, and immunohistochemical staining. Through bioinformatics prediction, we found that transforming growth factor β2 (TGFβ2) expression was upregulated in rats with BOO compared with normal bladder tissue. In vitro analyses using primary bladder smooth muscle cells (BSMCs) revealed that hydrogen peroxide (H2O2) induced TGFβ2 expression. Moreover, H2O2 induced epithelial-to-mesenchymal transition (EMT) by reducing E-cadherin, an endothelial marker and CK-18, a cytokeratin maker, and increasing mesenchymal markers, including N-cadherin, vimentin, and α-smooth muscle actin (α-SMA) levels. The downregulation of TGFβ2 expression in BSMCs using siRNA technology alleviated H2O2-induced changes in EMT marker expression. The findings of the study indicate that TGFβ2 plays a crucial role in BOO by participating in OS-induced EMT in BSMCs.

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TGFβ2介导氧化应激诱导的膀胱平滑肌上皮细胞向间质转化。
膀胱出口梗阻(BOO)是良性前列腺增生症的主要临床表现,是老年男性最常见的泌尿系统疾病,会导致相关的下尿路症状。据报道,虽然良性前列腺梗阻与全身氧化应激(OS)增加有关,但其根本机制仍不清楚。阐明这一机制是本研究的主要目的。本研究构建了一个 Sprague-Dawley 大鼠 BOO 模型,并将其用于尿动力学监测。采集大鼠膀胱组织并进行实时逆转录-定量聚合酶链反应(RT-qPCR)、组织学检查和免疫组化染色。通过生物信息学预测,我们发现与正常膀胱组织相比,患有膀胱癌的大鼠体内转化生长因子β2(TGFβ2)的表达上调。利用原代膀胱平滑肌细胞(BSMCs)进行的体外分析表明,过氧化氢(H2O2)可诱导 TGFβ2 的表达。此外,H2O2 还通过降低内皮标志物 E-cadherin和细胞角蛋白制造者 CK-18,以及增加间质标志物,包括 N-cadherin、波形蛋白和 α-平滑肌肌动蛋白(α-SMA)水平,诱导上皮向间质转化(EMT)。利用 siRNA 技术下调 BSMCs 中 TGFβ2 的表达可缓解 H2O2- 诱导的 EMT 标记表达变化。研究结果表明,TGFβ2 通过参与 OS 诱导的 BSMCs EMT,在 BOO 中发挥着至关重要的作用。
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来源期刊
CiteScore
3.70
自引率
4.80%
发文量
96
审稿时长
3 months
期刊介绍: In Vitro Cellular & Developmental Biology - Animal is a journal of the Society for In Vitro Biology (SIVB). Original manuscripts reporting results of research in cellular, molecular, and developmental biology that employ or are relevant to organs, tissue, tumors, and cells in vitro will be considered for publication. Topics covered include: Biotechnology; Cell and Tissue Models; Cell Growth/Differentiation/Apoptosis; Cellular Pathology/Virology; Cytokines/Growth Factors/Adhesion Factors; Establishment of Cell Lines; Signal Transduction; Stem Cells; Toxicology/Chemical Carcinogenesis; Product Applications.
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