LncRNA DGUOK-AS1 Promotes Cell Progression in Lung Squamous Cell Carcinoma by Regulation of miR-653-5p/SLC6A15 Axis.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Molecular Biotechnology Pub Date : 2025-02-01 Epub Date: 2024-02-26 DOI:10.1007/s12033-024-01088-8

Yan Teng, Shixia Li, Lijuan Wei, Chi Zhang, Lijuan Li, Shuang Wang, Jing Zhang, Jinchao Huang, Huan Zhang, Nan Wu, Juntian Liu

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Abstract

Long noncoding RNA (lncRNA) plays a key role in regulating cancer development. LncRNA deoxyguanosine kinase antisense RNA 1 (DGUOK-AS1) has been reported as a promoter in tumor. The work was designed to further investigate the mechanism of action of DGUOK-AS1 in lung squamous cell carcinoma (LUSC). DGUOK-AS1 level in LUSC cells was measured using RT-qPCR. Counting Kit-8 assays and colony forming assays were performed to evaluate LUSC cell viability and proliferation. Transwell assays were performed to detect cell migration and invasion. Luciferase reporter and RNA pulldown assays were used to verify the binding capacity of DGUOK-AS1 and miR-653-5p. RNA immunoprecipitation assays were performed to verify the relationship of DGUOK-AS1, miR-653-5p, and SLC6A15. DGUOK-AS1 was highly expressed in LUSC cells. DGUOK-AS1 knockdown suppressed LUSC cell proliferation, migration, and invasion. SLC6A15 was demonstrated to be targeted by miR-653-5p, and DGUOK-AS1 interacted with miR-653-5p to modulate SLC6A15 level in LUSC cells. Overexpression of SLC6A15 reversed the suppressive effects of DGUOK-AS1 knockdown on LUSC cell processes. In conclusion, DGUOK-AS1 promotes malignant behaviors of LUSC cells by upregulating SLC6A15 level through interaction with miR-653-5p.

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LncRNA DGUOK-AS1 通过调控 miR-653-5p/SLC6A15 轴促进肺鳞癌的细胞进展

长非编码 RNA（lncRNA）在调控癌症发展中发挥着关键作用。据报道，LncRNA脱氧鸟苷激酶反义RNA 1（DGUOK-AS1）是肿瘤的启动子。这项工作旨在进一步研究 DGUOK-AS1 在肺鳞癌（LUSC）中的作用机制。研究采用 RT-qPCR 法检测肺鳞状细胞癌细胞中 DGUOK-AS1 的水平。计数 Kit-8 试验和集落形成试验用于评估 LUSC 细胞的活力和增殖。透孔试验用于检测细胞迁移和侵袭。荧光素酶报告和 RNA pulldown 试验用于验证 DGUOK-AS1 和 miR-653-5p 的结合能力。RNA 免疫沉淀实验验证了 DGUOK-AS1、miR-653-5p 和 SLC6A15 的关系。DGUOK-AS1在LUSC细胞中高表达。敲除 DGUOK-AS1 可抑制 LUSC 细胞的增殖、迁移和侵袭。SLC6A15被证明是miR-653-5p的靶标，DGUOK-AS1与miR-653-5p相互作用，调节LUSC细胞中SLC6A15的水平。SLC6A15的过表达逆转了DGUOK-AS1敲除对LUSC细胞过程的抑制作用。总之，DGUOK-AS1通过与miR-653-5p相互作用上调SLC6A15水平，从而促进LUSC细胞的恶性行为。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.