Sophora flavescens alcohol extract ameliorates insomnia and promotes PI3K/AKT/BDNF signaling transduction in insomnia model rats.

IF 1.6 4区医学 Q4 NEUROSCIENCES Neuroreport Pub Date : 2024-03-20 Epub Date: 2024-02-18 DOI:10.1097/WNR.0000000000001999

Yanyan Wu, Chenhang Yao, Lan Zhang, Guoqing Wu

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Abstract

Active ingredient of Sophora flavescens is reported to promote non-rapid eye movement (NREM) sleep. However, the role of Sophora flavescens alcohol extract in insomnia is elusive, which is addressed in this study, together with the exploration on its potential mechanism. An insomnia model of rats was established by para-chlorophenylalanine induction and further treated with SFAE or Zaoren Anshen capsule (ZRAS; positive control drug). Sleep quality and sleep architecture of rats were evaluated by the sleep test, electroencephalogram and electromyogram. The levels of monoamine neurotransmitters in rat hypothalamus were determined using ELISA, and the transduction of the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/brain-derived neurotrophic factor (BDNF) signaling in the brain tissues of rats was examined by Western blot. SFAE and ZRAS increased the sleeping time and decreased the sleep latency of insomnia rats. SFAE reduced waking time and increased NREM and REM time, while changing power density of wakefulness, NREM sleep, and REM sleep in insomnia rats. SFAE and ZRAS upregulated levels of 5-hydroxytryptamine and 5-hydroxyindoleacetic acid, and downregulated those of norepinephrine and dopamine in insomnia rats. Besides, SFAE and ZRAS elevated BDNF expression as well as the ratios of phosphorylated (p)-PI3K/PI3K and p-AKT/AKT. The role of SFAE in insomnia model rats was similar with that of ZRAS. SFAE reduces insomnia and enhances the PI3K/AKT/BDNF signaling transduction in insomnia model rats, which can function as a drug candidate for insomnia.

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槐花醇提取物能改善失眠模型大鼠的失眠状况，并促进PI3K/AKT/BDNF信号转导。

据报道，洋槐的活性成分可促进非快速眼动（NREM）睡眠。然而，槐花醇提取物在失眠症中的作用尚不明确，本研究将探讨其潜在机制。本研究通过对氯苯丙氨酸诱导大鼠建立失眠模型，并进一步用 SFAE 或枣仁安神胶囊（ZRAS，阳性对照药物）治疗。通过睡眠测试、脑电图和肌电图评估大鼠的睡眠质量和睡眠结构。用酶联免疫吸附法测定大鼠下丘脑中单胺神经递质的水平，用Western印迹法检测大鼠脑组织中磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）/脑源性神经营养因子（BDNF）信号转导的情况。SFAE和ZRAS增加了失眠大鼠的睡眠时间，降低了睡眠潜伏期。SFAE减少了失眠大鼠的觉醒时间，增加了NREM和REM时间，同时改变了觉醒、NREM睡眠和REM睡眠的功率密度。SFAE和ZRAS能上调失眠大鼠体内5-羟色胺和5-羟基吲哚乙酸的水平，下调去甲肾上腺素和多巴胺的水平。此外，SFAE和ZRAS还能提高BDNF的表达以及磷酸化（p）-PI3K/PI3K和p-AKT/AKT的比率。SFAE对失眠模型大鼠的作用与ZRAS相似。SFAE能减轻失眠症模型大鼠的失眠症状，增强PI3K/AKT/BDNF信号转导，可作为治疗失眠症的候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.