Acute hypoxia causes glycolysis and lipid metabolism alterations with HSPs’ persistent activation in Leiocassis longirostris

IF 0.6 4区生物学 Q4 FISHERIES Ichthyological Research Pub Date : 2024-02-26 DOI:10.1007/s10228-024-00954-4

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Abstract

Hypoxia is one of the common stress factors encountered in intensive farming. Leiocassis longirostris is a commercially valuable fish with limited information on the response mechanism under acute hypoxia stress. Here, we detected the asphyxiation point and simulated acute hypoxia conditions, dissolved oxygen decreased, hypoxia maintained, and reoxygenation, and investigated molecular changes in the liver of L. longirostris. Results showed that the hypoxia-inducible factor-1 signaling pathway was enriched and hif1α, arnt, hph, epo, epor, and glut1 gene expression significantly increased. Peroxisome proliferator-activated receptor pathway with lipid metabolism-related genes (gk, scd, acsl, pgar, lpl, fabp3) was inhibited, while the glycolysis pathway with its related genes (pk, gapdhs, ldh, pfk, aldo, hk, gpi, pfkfb3) was activated under hypoxia. Furthermore, heat shock protein family gene expression increased significantly in both hypoxia treatments and the reoxygenation group, which suggests that immune response persistent activation was induced by acute hypoxic stress. This study provided valuable information for the metabolism and immune response in L. longirostris liver under acute hypoxia and reoxygenation conditions.

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急性缺氧导致糖酵解和脂质代谢改变，HSPs持续活化长尾鲤

摘要缺氧是集约化养殖中常见的应激因素之一。龙利鱼是一种具有商业价值的鱼类，但有关其在急性缺氧胁迫下的反应机制的信息却很有限。在此，我们检测了窒息点，模拟了急性缺氧条件、溶氧下降、缺氧维持和复氧，并研究了长尾鲈肝脏的分子变化。结果表明，缺氧诱导因子-1信号通路丰富，hif1α、arnt、hph、epo、epor和glut1基因表达显著增加。过氧化物酶体增殖激活受体通路中脂质代谢相关基因（gk、scd、acsl、pgar、lpl、fabp3）的表达受到抑制，而糖酵解通路中相关基因（pk、gapdhs、ldh、pfk、allo、hk、gpi、pfkfb3）的表达在缺氧条件下被激活。此外，热休克蛋白家族基因在缺氧处理和复氧组中的表达均显著增加，这表明急性缺氧应激诱导了免疫反应的持续激活。这项研究为研究急性缺氧和复氧条件下龙利鱼肝脏的新陈代谢和免疫反应提供了宝贵的信息。

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来源期刊

Ichthyological Research 生物-动物学

CiteScore

2.80

自引率

16.70%

发文量

审稿时长

3 months

期刊介绍： Ichthyological Research is an official journal of the Ichthyological Society of Japan and is published quarterly in January, April, July, and November. Ichthyological Research primarily publishes research papers on original work, either descriptive or experimental, that advances the understanding of the diversity of fishes. Ichthyological Research strives to cover all aspects of fish biology, including taxonomy, systematics, evolution, biogeography, ecology, ethology, genetics, morphology, and physiology.